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ARF1 Antibody (ARFS 3F1): sc-53167

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Datasheets
  • ARF1 Antibody (ARFS 3F1) is a mouse monoclonal IgG2a κ ARF1 antibody, cited in 4 publications, provided at 200 µg/ml
  • raised against the C-terminus of ARF1 of human origin
  • ARF1 Antibody (ARFS 3F1) is recommended for detection of ARF1 of mouse, rat and human origin by WB, IP and IHC(P)
  • Anti-ARF1 Antibody (ARFS 3F1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ARF1 Antibody (ARFS 3F1). This work is in progress.

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ARF1 Antibody (ARFS 3F1) is a mouse monoclonal IgG2a kappa light chain antibody that detects ARF1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunohistochemistry. Anti-ARF1 antibody (ARFS 3F1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ADP-ribosylation factor 1 (ARF1) protein is crucial for regulating vesicle trafficking and signal transduction within eukaryotic cells, playing a vital role in maintaining cellular organization and communication. Located primarily at the Golgi apparatus, ARF1 is essential for the formation of transport vesicles that shuttle proteins and lipids between cellular compartments, thereby ensuring proper cellular function and homeostasis. ARF1′s ability to interact with coat proteins and facilitate vesicle assembly underscores ARF1′s importance in intracellular transport processes. Additionally, ARF1 is involved in the activation of phospholipase D, which is critical for generating signaling molecules that modulate various cellular responses. The ARF protein family, which includes ARF1, ARF2, and ARF3 as class I members, shares a conserved structure and gene organization, highlighting the evolutionary significance of these proteins in cellular regulation.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

ARF1 Antibody (ARFS 3F1)

sc-53167
200 µg/ml
$316.00

ARF1 Antibody (ARFS 3F1) AC

sc-53167 AC
500 µg/ml, 25% agarose
$416.00

ARF1 Antibody (ARFS 3F1) HRP

sc-53167 HRP
200 µg/ml
$316.00

ARF1 Antibody (ARFS 3F1) FITC

sc-53167 FITC
200 µg/ml
$330.00

ARF1 Antibody (ARFS 3F1) PE

sc-53167 PE
200 µg/ml
$343.00

ARF1 Antibody (ARFS 3F1) Alexa Fluor® 488

sc-53167 AF488
200 µg/ml
$357.00

ARF1 Antibody (ARFS 3F1) Alexa Fluor® 546

sc-53167 AF546
200 µg/ml
$357.00

ARF1 Antibody (ARFS 3F1) Alexa Fluor® 594

sc-53167 AF594
200 µg/ml
$357.00

ARF1 Antibody (ARFS 3F1) Alexa Fluor® 647

sc-53167 AF647
200 µg/ml
$357.00

ARF1 Antibody (ARFS 3F1) Alexa Fluor® 680

sc-53167 AF680
200 µg/ml
$357.00

ARF1 Antibody (ARFS 3F1) Alexa Fluor® 790

sc-53167 AF790
200 µg/ml
$357.00

Can ARF (ARFS 3F1): sc-53167 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Professor Griffin
Thank you for your question. Yes, ARF (ARFS 3F1): sc-53167 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
  • y_2025, m_11, d_13, h_10CST
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Rated 1 out of 5 by from WB for mouse brain was not workedWB for mouse brain was not worked. There were some positive control using another anti-ARF antibodies.
Date published: 2017-12-09
Rated 5 out of 5 by from It works well for WBI dilute the antibody at 1:1000 and incubate at 4℃ overnight.
Date published: 2017-09-18
Rated 5 out of 5 by from Strong band of interest detected in WB inStrong band of interest detected in WB in human ARF1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Positive result in WB in CakiPositive result in WB in Caki-1 whole cell lysate. -SCBT QC
Date published: 2015-01-28
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmicProduced excellent immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-01-02
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ARF1 Antibody (ARFS 3F1) is rated 4.2 out of 5 by 5.
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