ARD Antibody (H-3): sc-398325

ARD Antibody (H-3) is a mouse monoclonal IgM antibody that detects ARD protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ARD antibody (H-3) is available as the non-conjugated form. ARD protein, also known as acireductone dioxygenase, ADI1, APL1, SIPL, SIP-L, or MTCBP1, consists of 179 amino acids and is primarily localized in the nucleus and cytoplasmic side of the cell membrane. This localization enables ARD protein to play a pivotal role in cellular metabolism, particularly in the L-methionine biosynthetic pathway, where ARD protein catalyzes the conversion of (S)-methyl-5-thio-alpha-D-ribose 1-phosphate to L-methionine using nickel as a cofactor. L-methionine serves as a building block for proteins and is involved in various metabolic processes, including methylation reactions that regulate gene expression and protein function. ARD protein interacts with matrix metalloproteinase MT-MMP-1, suggesting a potential role in modulating cell migration, which is crucial in processes such as wound healing and cancer metastasis. Multiple isoforms of ARD protein exist due to alternative splicing, contributing to functional diversity and regulatory mechanisms within different tissues.

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ARD Antibody (H-3) References:

1. Identification of a hepatic factor capable of supporting hepatitis C virus replication in a nonpermissive cell line.  |  Yeh, CT., et al. 2001. J Virol. 75: 11017-24. PMID: 11602742
2. Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors.  |  Uekita, T., et al. 2004. J Biol Chem. 279: 12734-43. PMID: 14718544
3. Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas.  |  Yamada, S., et al. 2004. Oncogene. 23: 5901-11. PMID: 15221005
4. Membrane-type 1 matrix metalloproteinase cytoplasmic tail binding protein-1 (MTCBP-1) acts as an eukaryotic aci-reductone dioxygenase (ARD) in the methionine salvage pathway.  |  Hirano, W., et al. 2005. Genes Cells. 10: 565-74. PMID: 15938715
5. Regulated nucleo-cytoplasmic shuttling of human aci-reductone dioxygenase (hADI1) and its potential role in mRNA processing.  |  Gotoh, I., et al. 2007. Genes Cells. 12: 105-17. PMID: 17212658
6. Expression and function of the human androgen-responsive gene ADI1 in prostate cancer.  |  Oram, SW., et al. 2007. Neoplasia. 9: 643-51. PMID: 17786183

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ARD Antibody (H-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-ARD Antibody (H-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ARD Antibody (H-3) Specifications

Alternate Product Name: ARD

Product Clone Name: H-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 52-69

Epitope Target: ARD

Epitope Species: human

Product Reactivity/Detection: ARD

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for ARD Antibody (H-3): P: Blocking Peptide