Aquaporin 4/AQP4 Double Nickase Plasmid (h): sc-400258-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aquaporin 4/AQP4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Aquaporin 4/AQP4 Double Nickase Plasmid (h) and Aquaporin 4/AQP4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AQP4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aquaporin 4/AQP4 Antibody (4/18): sc-32739
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aquaporin 4/AQP4 Double Nickase Plasmid (h) | sc-400258-NIC | 20 µg | $410.00 | |||
Aquaporin 4/AQP4 Double Nickase Plasmid (h2) | sc-400258-NIC-2 | 20 µg | $410.00 |
Human AQP4 encodes aquaporin-4, a major water channel enriched at astrocyte endfeet that supports rapid bidirectional water movement across the plasma membrane and helps maintain brain water homeostasis. By coupling with ion flux and neurotransmission, AQP4 contributes to astrocyte volume regulation, extracellular space dynamics, and glymphatic transport processes that influence clearance of interstitial solutes. Its polarized membrane localization is coordinated with perivascular scaffolding complexes and is tightly linked to blood–brain barrier physiology and osmotic stress responses. Dysregulation of AQP4 expression or localization has been associated with neuroinflammatory and demyelinating disorders, cerebral edema, and altered seizure susceptibility, making it a key target for mechanistic studies of CNS fluid balance and glial biology.
Aquaporin 4/AQP4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AQP4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AQP4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AQP4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AQP4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.