Aquaporin 3/AQP3 Double Nickase Plasmid (h): sc-400636-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aquaporin 3/AQP3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Aquaporin 3/AQP3 Double Nickase Plasmid (h) and Aquaporin 3/AQP3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AQP3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aquaporin 3/AQP3 Antibody (F-1): sc-518001
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aquaporin 3/AQP3 Double Nickase Plasmid (h) | sc-400636-NIC | 20 µg | $410.00 | |||
Aquaporin 3/AQP3 Double Nickase Plasmid (h2) | sc-400636-NIC-2 | 20 µg | $410.00 |
AQP3 encodes aquaporin-3, a membrane channel that facilitates transcellular transport of water, glycerol, and other small solutes, thereby influencing cell volume regulation, osmotic homeostasis, and cellular metabolism. Through glycerol permeability, AQP3 links membrane transport to energy balance and redox-related processes that impact proliferation, migration, and stress responses in epithelial and immune contexts. AQP3 expression and localization are studied in barrier tissues such as skin and mucosa, where altered channel activity can modulate hydration and inflammatory signaling. Dysregulated AQP3 has been associated with pathological remodeling and tumor biology, making it a useful target for mechanistic studies of transport-driven signaling and disease-relevant phenotypes.
Aquaporin 3/AQP3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AQP3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AQP3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AQP3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AQP3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.