Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Aquaporin 3/AQP3 CRISPR Activation Plasmid (h): sc-400636-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aquaporin 3/AQP3 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Aquaporin 3/AQP3 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Aquaporin 3/AQP3 CRISPR Activation Plasmid (h) and Aquaporin 3/AQP3 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the AQP3 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aquaporin 3/AQP3 Antibody (F-1): sc-518001
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aquaporin 3/AQP3 CRISPR Activation Plasmid (h) | sc-400636-ACT | 20 µg | $397.00 |
Human AQP3 encodes aquaporin 3, a membrane channel that facilitates water and glycerol transport across epithelial and immune cell membranes, influencing osmotic balance and cellular metabolism. By regulating glycerol flux, AQP3 can affect energy homeostasis, redox status, and signaling processes linked to membrane dynamics, proliferation, and barrier function. Its activity is studied in contexts such as epithelial differentiation, skin hydration, and immune cell migration, where altered expression has been reported in inflammatory conditions and multiple cancer types. AQP3 is also used as a marker and functional node in pathways connecting solute transport to cell stress responses and microenvironmental adaptation.
Aquaporin 3/AQP3 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous AQP3 expression without altering the underlying DNA sequence.
Aquaporin 3/AQP3 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the AQP3 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the AQP3 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous Aquaporin 3/AQP3 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native AQP3 locus and enabling the study of Aquaporin 3/AQP3-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of Aquaporin 3/AQP3 pathway restoration in tumor cells with silenced or reduced AQP3 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.