Aquaporin 1/AQP1 Double Nickase Plasmid (m): sc-419172-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aquaporin 1/AQP1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Aquaporin 1/AQP1 Double Nickase Plasmid (m) and Aquaporin 1/AQP1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Aqp1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aquaporin 1/AQP1 Antibody (B-11): sc-25287
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aquaporin 1/AQP1 Double Nickase Plasmid (m) | sc-419172-NIC | 20 µg | $410.00 | |||
Aquaporin 1/AQP1 Double Nickase Plasmid (m2) | sc-419172-NIC-2 | 20 µg | $410.00 |
Mouse Aqp1 encodes aquaporin 1 (AQP1), a tetrameric membrane channel that mediates rapid, selective water flux across plasma membranes and supports osmotic homeostasis. AQP1 is highly expressed in microvascular endothelium and epithelial tissues, where it contributes to transepithelial fluid transport, cell volume regulation, and physiological barrier function. Through its effects on membrane permeability and hydrostatic/osmotic gradients, AQP1 influences processes such as edema formation, angiogenic responses, and fluid secretion. Altered AQP1 expression or localization has been associated with renal concentrating defects, vascular dysfunction, and dysregulated tissue fluid balance relevant to inflammatory and neoplastic microenvironments.
Aquaporin 1/AQP1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Aqp1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Aqp1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Aqp1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Aqp1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.