apoOL Double Nickase Plasmid (h): sc-414464-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- apoOL Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- apoOL Double Nickase Plasmid (h) and apoOL Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting APOOL. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: apoOL Antibody (G-6): sc-390958
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
apoOL Double Nickase Plasmid (h) | sc-414464-NIC | 20 µg | $410.00 |
APOOL encodes apoOL, a mitochondria-associated protein enriched in the inner membrane that contributes to cardiolipin-dependent membrane organization and mitochondrial cristae architecture. Through interactions with the MICOS complex and related lipid remodeling processes, apoOL influences oxidative phosphorylation efficiency, mitochondrial dynamics, and susceptibility to stress-induced bioenergetic failure. Perturbation of APOOL expression or mitochondrial localization has been linked to altered respiration, reactive oxygen species handling, and defects in organelle ultrastructure, processes frequently implicated in neurodegeneration, cardiomyopathy, and metabolic dysfunction. Accordingly, APOOL is studied in pathways governing mitochondrial membrane homeostasis, apoptosis sensitivity, and cellular adaptation to energetic stress.
apoOL Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the APOOL locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within APOOL. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt APOOL function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of APOOL-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.