apoH Antibody (1D2): sc-58244

apoH Antibody (1D2) is a mouse monoclonal IgG1 antibody that detects apoH of human origin by western blotting (WB), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-apoH antibody (1D2) is available as the non-conjugated isotype antibody form. Human apolipoprotein H (apoH), also known as β 2-glycoprotein I, activated protein C binding protein, or APC inhibitor, is a five-domain plasma membrane-adhesion protein that plays a crucial role in various physiological processes, including blood coagulation and immune response regulation. One of the significant aspects of apoH is its ability to undergo post-translational modifications, particularly glycosylation, which enhances stability and functionality in the bloodstream. These modifications are essential as they influence apoH′s interaction with other molecules, including a role as a cofactor for serum auto-antibodies in antiphospholipid syndrome, linking apoH to conditions such as thrombosis, lupus erythematosus, and recurrent fetal loss. Additionally, apoH is involved in the clearance of apoptotic bodies from circulation, highlighting importance in maintaining homeostasis. The apoH gene is located on human chromosome 17q23-24, and apoH is synthesized by hepatocytes, where apoH associates with plasma lipoproteins. Notably, apoH exhibits genetically determined structural polymorphisms, including three alleles (apoH 1, apoH 2, and apoH 3), which may contribute to individual variations in disease susceptibility and lipid metabolism. Furthermore, apoH has been shown to inhibit the translocation of cholesterol from extracellular pools to macrophages, thereby reducing cellular cholesterol accumulation and suggesting a protective role against atherosclerosis.

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apoH Antibody (1D2) References:

1. Adhesion mechanism of human beta(2)-glycoprotein I to phospholipids based on its crystal structure.  |  Bouma, B., et al. 1999. EMBO J. 18: 5166-74. PMID: 10508150
2. Crystal structure of human beta2-glycoprotein I: implications for phospholipid binding and the antiphospholipid syndrome.  |  Schwarzenbacher, R., et al. 1999. EMBO J. 18: 6228-39. PMID: 10562535
3. Evidence for inhibition of low density lipoprotein oxidation and cholesterol accumulation by apolipoprotein H (beta2-glycoprotein I).  |  Lin, KY., et al. 2001. Life Sci. 69: 707-19. PMID: 11476192
4. Complete nucleotide and deduced amino acid sequence of human beta 2-glycoprotein I.  |  Steinkasserer, A., et al. 1991. Biochem J. 277 (Pt 2): 387-91. PMID: 1650181
5. Nucleotide sequence and expression of the human gene encoding apolipoprotein H (beta 2-glycoprotein I).  |  Mehdi, H., et al. 1991. Gene. 108: 293-8. PMID: 1748314
6. APOH is increased in the plasma and liver of type 2 diabetic patients with metabolic syndrome.  |  Castro, A., et al. 2010. Atherosclerosis. 209: 201-5. PMID: 19878946
7. Exosome-Contained APOH Associated With Antiphospholipid Syndrome.  |  Tan, Y., et al. 2021. Front Immunol. 12: 604222. PMID: 34040601
8. The association of APOH and NCF1 polymorphisms on susceptibility to recurrent pregnancy loss in women with antiphospholipid syndrome.  |  Deng, X., et al. 2023. J Assist Reprod Genet. 40: 1703-1712. PMID: 37243946
9. Fructus arctii mitigates diabetic nephropathy via the Apoh/PPAR-γ pathway.  |  Zhang, N., et al. 2025. Mol Immunol. 181: 18-28. PMID: 40056629
10. Qualitative analysis of the carbohydrate composition of apolipoprotein H.  |  Gambino, R., et al. 1997. J Protein Chem. 16: 205-12. PMID: 9155091
11. Influence of APOH protein polymorphism on apoH levels in normal and diabetic subjects.  |  Ruiu, G., et al. 1997. Clin Genet. 52: 167-72. PMID: 9377806
12. Structure of the human beta2-glycoprotein I (apolipoprotein H) gene.  |  Okkels, H., et al. 1999. Eur J Biochem. 259: 435-40. PMID: 9914524

The preferred apoH antibody is apoH Antibody (C-2): sc-515677.