apoC-III CRISPR/Cas9 KO Plasmid (m): sc-419165
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- apoC-III CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the apoC-III genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
apoC-III CRISPR/Cas9 KO Plasmid (m) | sc-419165 | 20 µg | $397.00 |
Overview
Mouse Apoc3 encodes apolipoprotein C-III (apoC-III), a small secreted apolipoprotein that modulates plasma triglyceride homeostasis by regulating the metabolism and clearance of triglyceride-rich lipoproteins. apoC-III influences lipoprotein lipase–dependent lipolysis and hepatic uptake pathways, shaping VLDL and chylomicron remnant handling and downstream lipid flux. Through these processes, Apoc3 activity intersects with systemic energy balance and inflammatory signaling associated with dyslipidemia-related phenotypes. Altered apoC-III levels are widely used as a molecular readout in studies of hypertriglyceridemia, atherosclerosis susceptibility, and cardiometabolic risk mechanisms.
apoC-III CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Apoc3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Apoc3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Apoc3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish apoC-III protein expression.
This CRISPR knockout system enables efficient generation of Apoc3-deficient cell models for investigation of apoC-III signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Apoc3 exon(s) critical for apoC-III function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Apoc3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by apoC-III CRISPR/Cas9 KO Plasmid (m) and apoC-III CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Apoc3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by apoC-III HDR Plasmid (m) and apoC-III HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Apoc3 homology arms to support homology-directed repair at defined Apoc3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.