apoB Antibody (A-6): sc-393636

apoB Antibody (A-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects apoB in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). apoB Antibody (A-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. apoB plays a crucial role in lipid metabolism and is essential for the formation and transport of lipoproteins, which are vital for the distribution of cholesterol and triglycerides throughout the body. Two primary isoforms of apoB exist: apoB-100, synthesized in the liver and serving as a structural component of very-low-density lipoprotein particles, and apoB-48, produced in the small intestine, which is crucial for chylomicron formation. The differential expression of these isoforms results from post-transcriptional editing of a common apoB mRNA, a process regulated by the enzyme APOBEC1. This editing allows for the generation of distinct protein forms that fulfill specific physiological roles, highlighting apoB′s importance in maintaining lipid homeostasis and overall cardiovascular health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

apoB Antibody (A-6) References:

1. The molecular mechanism for the assembly and secretion of ApoB-100-containing lipoproteins.  |  Borén, J., et al. 1991. Prog Lipid Res. 30: 205-18. PMID: 1823939
2. ApoB-100-containing lipoproteins are major carriers of 3-iodothyronamine in circulation.  |  Roy, G., et al. 2012. J Biol Chem. 287: 1790-800. PMID: 22128163
3. Novel functional APOB mutations outside LDL-binding region causing familial hypercholesterolaemia.  |  Alves, AC., et al. 2014. Hum Mol Genet. 23: 1817-28. PMID: 24234650
4. Autoantibodies Against ApoB-100 as a New Marker of Coronary Vulnerable Plaque.  |  Egusa, G. 2021. J Atheroscler Thromb. 28: 1020-1022. PMID: 33455997
5. Homozygous familial hypercholesterolemia due to APOB genetic variant with unusual clinical course.  |  Chlebus, K., et al. 2021. Kardiol Pol. 79: 1030-1031. PMID: 34125946
6. The importance of APOB gene expression as a marker of atherosclerosis severity in coronary vessels.  |  Owczarek, J., et al. 2023. Pol Arch Intern Med. 133: PMID: 37548372
7. ApoA-I and ApoB levels, and ApoB-to-ApoA-I ratio as candidate pre-treatment biomarkers of pathomorphological response to neoadjuvant therapy in gastric and esophago-gastric junction adenocarcinoma.  |  Nurczyk, K., et al. 2023. Pol Przegl Chir. 96: 44-49. PMID: 38629280
8. High LDL Particle and APOB Concentrations in Patients With Adrenal Cortical Adenomas.  |  Sandooja, R., et al. 2024. J Clin Endocrinol Metab. 110: 195-207. PMID: 38870260
9. The assembly and secretion of apoB-100-containing lipoproteins.  |  Borén, J., et al. 1993. Biochem Soc Trans. 21: 487-93. PMID: 8359516
10. Cellular consequences of the association of apoB lipoproteins with proteoglycans. Potential contribution to atherogenesis.  |  Hurt-Camejo, E., et al. 1997. Arterioscler Thromb Vasc Biol. 17: 1011-7. PMID: 9194748

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-apoB Antibody (A-6) has 29 citations in a variety of scientific publications.

Primary antibodies like Anti-apoB Antibody (A-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

apoB Antibody (A-6) Specifications

Alternate Product Name: apoB

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: apoB

Epitope Species: human

Product Reactivity/Detection: apoB

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 29 citations in various scientific publications

Available Conjugates/Forms for apoB Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred apoB antibody is apoB Antibody (C1.4): sc-13538.