apoA-IV Antibody (G-8): sc-374543

apoA-IV Antibody (G-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects apoA-IV protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). apoA-IV monoclonal antibody (G-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. apoA-IV protein, encoded by the human apoA-IV gene, is a 396 amino acid preprotein that plays a crucial role in lipid metabolism. After proteolytic processing, apoA-IV is secreted from the intestine in association with chylomicron particles, where apoA-IV functions as a potent activator of lecithin cholesterol acyltransferase (LCAT), an enzyme essential for the formation of cholesteryl esters in plasma. This activation is vital for the reverse transport of cholesterol, a process that helps maintain lipid homeostasis and prevent atherosclerosis. Additionally, apoA-IV is involved in regulating appetite and energy expenditure, highlighting apoA-IV′s importance in metabolic health. apoA-IV′s ability to interact with other apolipoproteins and lipoproteins further underscores apoA-IV′s significance in cardiovascular and metabolic diseases, making anti-apoA-IV antibody (G-8) an invaluable tool for researchers studying these critical pathways.

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apoA-IV Antibody (G-8) References:

1. Phospholipid transfer protein gene knock-out mice have low high density lipoprotein levels, due to hypercatabolism, and accumulate apoA-IV-rich lamellar lipoproteins.  |  Qin, S., et al. 2000. J Lipid Res. 41: 269-76. PMID: 10681411
2. Human ApoA-IV overexpression in transgenic mice induces cAMP-stimulated cholesterol efflux from J774 macrophages to whole serum.  |  Fournier, N., et al. 2000. Arterioscler Thromb Vasc Biol. 20: 1283-92. PMID: 10807744
3. GPI-specific phospholipase D associates with an apoA-I- and apoA-IV-containing complex.  |  Deeg, MA., et al. 2001. J Lipid Res. 42: 442-51. PMID: 11254757
4. Butyrate stimulates ApoA-IV-containing lipoprotein secretion in differentiated Caco-2 cells: role in cholesterol efflux.  |  Nazih, H., et al. J Cell Biochem. 83: 230-8. PMID: 11573240
5. Increased plasma apoA-IV level is a marker of abnormal postprandial lipemia: a study in normoponderal and obese subjects.  |  Vergès, B., et al. 2001. J Lipid Res. 42: 2021-9. PMID: 11734575
6. Plasma distribution of apoA-IV in patients with coronary artery disease and healthy controls.  |  Ezeh, B., et al. 2003. J Lipid Res. 44: 1523-9. PMID: 12777472
7. apoA-IV tagged with the ER retention signal KDEL perturbs the intracellular trafficking and secretion of apoB.  |  Gallagher, JW., et al. 2004. J Lipid Res. 45: 1826-34. PMID: 15258202
8. Response of ApoA-IV in pigs to long-term increased dietary oil intake and to the degree of unsaturation of the fatty acids.  |  Navarro, MA., et al. 2004. Br J Nutr. 92: 763-9. PMID: 15533264
9. ApoA-IV promotes the biogenesis of apoA-IV-containing HDL particles with the participation of ABCA1 and LCAT.  |  Duka, A., et al. 2013. J Lipid Res. 54: 107-15. PMID: 23132909
10. ApoA-IV: current and emerging roles in intestinal lipid metabolism, glucose homeostasis, and satiety.  |  Kohan, AB., et al. 2015. Am J Physiol Gastrointest Liver Physiol. 308: G472-81. PMID: 25591862
11. Clinical ApoA-IV amyloid is associated with fibrillogenic signal sequence.  |  Canetti, D., et al. 2021. J Pathol. 255: 311-318. PMID: 34331462
12. The apolipoprotein A-I/C-III/A-IV gene cluster: ApoC-III and ApoA-IV expression is regulated by two common enhancers.  |  Vergnes, L., et al. 1997. Biochim Biophys Acta. 1348: 299-310. PMID: 9366246

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-apoA-IV Antibody (G-8) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-apoA-IV Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

apoA-IV Antibody (G-8) Specifications

Alternate Product Name: APOA4

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: apoA-IV

Epitope Species: human

Product Reactivity/Detection: apoA-IV

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for apoA-IV Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin