APNG CRISPR/Cas9 KO Plasmid (m): sc-435240
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- APNG CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the APNG genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APNG CRISPR/Cas9 KO Plasmid (m) | sc-435240 | 20 µg | $397.00 |
Overview
Mouse Mpg encodes alkylpurine DNA N-glycosylase (APNG), a base excision repair enzyme that recognizes and excises a range of alkylated and deaminated purine lesions, including 3-methyladenine and hypoxanthine. By initiating removal of damaged bases, APNG helps maintain replication fidelity and limits mutagenesis arising from endogenous metabolism and environmental genotoxins. APNG activity interfaces with core BER factors such as APEX1, POLβ, and XRCC1 to complete lesion processing and strand restoration. Altered MPG/APNG function has been linked to genome instability phenotypes relevant to carcinogenesis research and sensitivity to DNA alkylation stress in cell and mouse models.
APNG CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mpg gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mpg together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mpg open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish APNG protein expression.
This CRISPR knockout system enables efficient generation of Mpg-deficient cell models for investigation of APNG signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mpg exon(s) critical for APNG function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mpg genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by APNG CRISPR/Cas9 KO Plasmid (m) and APNG CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mpg locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by APNG HDR Plasmid (m) and APNG HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mpg homology arms to support homology-directed repair at defined Mpg target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.