APC7 Double Nickase Plasmid (m): sc-425084-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- APC7 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- APC7 Double Nickase Plasmid (m) and APC7 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Anapc7. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: APC7 Antibody (A-6): sc-365649
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
APC7 Double Nickase Plasmid (m) | sc-425084-NIC | 20 µg | $410.00 | |||
APC7 Double Nickase Plasmid (m2) | sc-425084-NIC-2 | 20 µg | $410.00 |
Mouse Anapc7 encodes APC7, a core tetratricopeptide repeat (TPR) subunit of the anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase that drives timely progression through mitosis and G1 by targeting key cell-cycle regulators for proteasomal degradation. APC7 supports APC/C assembly and coactivator interactions that coordinate substrate recognition during the spindle assembly checkpoint, anaphase onset, and mitotic exit. Disruption of APC/C function can destabilize genome maintenance by impairing chromatid separation and checkpoint fidelity, linking APC/C subunits to aneuploidy-associated phenotypes in proliferative tissues. Anapc7 is therefore frequently studied in pathways governing ubiquitin-mediated proteolysis, chromosome segregation, and cell-cycle control in mouse models.
APC7 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Anapc7 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Anapc7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Anapc7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Anapc7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.