AP4A Hydrolase CRISPR/Cas9 KO Plasmid (r): sc-437371
- Target species: rat
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AP4A Hydrolase CRISPR/Cas9 Knockout (KO) Plasmid (r) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AP4A Hydrolase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AP4A Hydrolase Antibody (F-5): sc-271410
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AP4A Hydrolase CRISPR/Cas9 KO Plasmid (r) | sc-437371 | 20 µg | $397.00 |
Overview
AP4A hydrolase is a nucleotide-metabolizing enzyme that hydrolyzes diadenosine tetraphosphate (Ap4A), a pleiotropic alarmone-like signaling molecule whose abundance rises during cellular stress. By controlling Ap4A turnover, the enzyme contributes to nucleotide homeostasis and influences downstream processes linked to stress adaptation, redox balance, and regulation of nucleic acid–binding proteins. Altered Ap4A metabolism has been associated with changes in proliferation, inflammatory signaling, and neuronal function, making AP4A hydrolase relevant to mechanistic studies of cardiometabolic, neurobiology, and immune-related phenotypes in rat models. Its activity intersects broadly with pathways governing RNA/DNA processing and cellular stress responses where dinucleotide second messengers act as modulators.
AP4A Hydrolase CRISPR/Cas9 KO Plasmid (r) is a pool of plasmids designed for targeted disruption of the gene in rat cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AP4A Hydrolase protein expression.
This CRISPR knockout system enables efficient generation of -deficient cell models for investigation of AP4A Hydrolase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting exon(s) critical for AP4A Hydrolase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AP4A Hydrolase CRISPR/Cas9 KO Plasmid (r) and AP4A Hydrolase CRISPR/Cas9 KO Plasmid (r2) target distinct sites within the locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AP4A Hydrolase HDR Plasmid (r) and AP4A Hydrolase HDR Plasmid (r2) contain a puromycin resistance cassette and an RFP reporter flanked by homology arms to support homology-directed repair at defined target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.