AP-2μ1 Antibody (D-7): sc-515920

AP-2μ1 Antibody (D-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects AP-2 mu 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AP-2μ1 (D-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AP-2 mu 1, consisting of 435 amino acids, plays a crucial role in the endocytic pathway by linking Clathrin to receptors in coated vesicles, facilitating membrane protein and signaling molecule internalization. This process maintains cellular homeostasis and regulates various physiological functions, including nutrient uptake and receptor recycling. AP-2 mu 1′s location at the plasma membrane and interaction with Clathrin-coated pits underscore AP-2 mu 1′s importance in vesicle-mediated transport, which enables cellular communication and response to external stimuli. Understanding AP-2 mu 1 dynamics and associated complexes can provide insights into endocytosis mechanisms and implications in diseases where these processes are disrupted.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

AP-2μ1 Antibody (D-7) References:

1. Genomic structure and chromosome mapping of the genes encoding clathrin-associated adaptor medium chains mu1A (Ap1m1) and mu1B (Ap1m2).  |  Nakatsu, F., et al. 1999. Cytogenet Cell Genet. 87: 53-8. PMID: 10640811
2. Distinct and redundant functions of mu1 medium chains of the AP-1 clathrin-associated protein complex in the nematode Caenorhabditis elegans.  |  Shim, J., et al. 2000. Mol Biol Cell. 11: 2743-56. PMID: 10930467
3. Similar subunit interactions contribute to assembly of clathrin adaptor complexes and COPI complex: analysis using yeast three-hybrid system.  |  Takatsu, H., et al. 2001. Biochem Biophys Res Commun. 284: 1083-9. PMID: 11409905
4. Adaptins: the final recount.  |  Boehm, M. and Bonifacino, JS. 2001. Mol Biol Cell. 12: 2907-20. PMID: 11598180
5. [Role of the adaptins, dynamin like GTP-ases and Rab proteins in metabolic disorders and various infections].  |  Kierczak, M., et al. 2003. Postepy Hig Med Dosw. 57: 727-37. PMID: 15002167
6. The AP-1 μ adaptin is required for KNOLLE localization at the cell plate to mediate cytokinesis in Arabidopsis.  |  Teh, OK., et al. 2013. Plant Cell Physiol. 54: 838-47. PMID: 23543752
7. Arabidopsis μ-adaptin subunit AP1M of adaptor protein complex 1 mediates late secretory and vacuolar traffic and is required for growth.  |  Park, M., et al. 2013. Proc Natl Acad Sci U S A. 110: 10318-23. PMID: 23733933
8. Expression levels of SIX1, ME2, and AP2M1 in adenoid cystic carcinoma and mucoepidermoid carcinoma.  |  Wu, CC., et al. 2020. Oral Dis. 26: 1687-1695. PMID: 32564485
9. AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.  |  Ganapathy, AS., et al. 2022. Autophagy. 18: 2086-2103. PMID: 34964704
10. Phosphorylation of ADAPTOR PROTEIN-2 μ-adaptin by ADAPTOR-ASSOCIATED KINASE1 regulates the tropic growth of Arabidopsis roots.  |  Siao, W., et al. 2023. Plant Cell. 35: 3504-3521. PMID: 37440281
11. Identification of two new mu-adaptin-related proteins, mu-ARP1 and mu-ARP2.  |  Wang, X. and Kilimann, MW. 1997. FEBS Lett. 402: 57-61. PMID: 9013859
12. Identification and characterization of novel clathrin adaptor-related proteins.  |  Takatsu, H., et al. 1998. J Biol Chem. 273: 24693-700. PMID: 9733768

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AP-2μ1 Antibody (D-7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-AP-2μ1 Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AP-2μ1 Antibody (D-7) Specifications

Alternate Product Name: AP-2μ1

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 16-38

Epitope Target: AP-2µ1

Epitope Species: human

Product Reactivity/Detection: AP-2µ1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for AP-2μ1 Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin