AOX1 Double Nickase Plasmid (h): sc-403997-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AOX1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AOX1 Double Nickase Plasmid (h) and AOX1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AOX1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AOX1 Antibody (D-8): sc-365291
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AOX1 Double Nickase Plasmid (h) | sc-403997-NIC | 20 µg | $410.00 | |||
AOX1 Double Nickase Plasmid (h2) | sc-403997-NIC-2 | 20 µg | $410.00 |
Aldehyde oxidase 1 (AOX1) is a cytosolic molybdo-flavoenzyme that catalyzes the oxidation of diverse aldehydes and N-heterocyclic compounds, contributing to phase I xenobiotic metabolism and cellular redox balance. By generating reactive oxygen species during substrate turnover, AOX1 can influence oxidative stress–responsive signaling and downstream inflammatory programs. AOX1 expression is prominent in liver and other metabolically active tissues, where it intersects with broader drug metabolism and detoxification networks. Altered AOX1 activity or regulation has been associated with inter-individual variation in xenobiotic handling and has been explored in contexts of hepatic dysfunction and oxidative stress–linked pathophysiology.
AOX1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AOX1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AOX1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AOX1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AOX1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.