anti-Cy5 Antibody (B-2): sc-166896
- anti-Cy5 Antibody (B-2) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
- anti-Cy5 Antibody (B-2) is recommended for detection of Cy5 by WB, IP, IF and ELISA
- Anti-anti-Cy5 Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for anti-Cy5 Antibody (B-2) for WB applications. These reagents are now offered in bundles with anti-Cy5 Antibody (B-2) (see ordering information below).
QUICK LINKS
SEE ALSO...
Anti-Cy5 Antibody (B-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Cy5 in non-species reactive applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Cy5 antibody (B-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Cy5, a member of the cyanine dye family, plays a crucial role in biomedical imaging due to its ability to emit fluorescence across a wide spectrum, from infrared to ultraviolet, which enhances the sensitivity and specificity of imaging techniques. The structural characteristics of Cy5, including its partially saturated indole nitrogen heterocyclic nucleus and the polyalkene bridge, contribute to unique optical properties, making Cy5 an invaluable tool in various applications, such as increasing the sensitivity range of photographic emulsions and facilitating the visualization of biological processes. Anti-Cy5 antibody (B-2) can be utilized in a linker system to bind cyanine dyes, allowing for the development of advanced imaging techniques that can target specific enzymes at their active sites, thereby enhancing the overall efficacy of experimental protocols.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
anti-Cy5 Antibody (B-2) References:
- Verification of specific G-quadruplex structure by using a novel cyanine dye supramolecular assembly: II. The binding characterization with specific intramolecular G-quadruplex and the recognizing mechanism. | Yang, Q., et al. 2010. Nucleic Acids Res. 38: 1022-33. PMID: 19933263
- Synthesis and spectroscopic and DNA-binding properties of fluorogenic acridine-containing cyanine dyes. | Mahmood, T., et al. 2010. J Org Chem. 75: 204-7. PMID: 19954141
- Fluorescence lifetime properties of near-infrared cyanine dyes in relation to their structures. | Lee, H., et al. 2008. J Photochem Photobiol A Chem. 200: 438-444. PMID: 20016664
- Fast assembly of cyanine dyes into aggregates onto [6,6]-phenyl C61-butyric acid methyl ester surfaces from organic solvents. | Heier, J., et al. 2010. Langmuir. 26: 3955-61. PMID: 20073528
- Electronic and vibrational coherence dynamics in a cyanine dye studied using a few-cycle pulsed laser. | Wang, Y. and Kobayashi, T. 2010. Chemphyschem. 11: 889-96. PMID: 20135666
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
anti-Cy5 Antibody (B-2) | sc-166896 | 200 µg/ml | $322.00 | |||
anti-Cy5 Antibody (B-2): m-IgG Fc BP-HRP Bundle | sc-527194 | 200 µg Ab; 10 µg BP | $361.00 | |||
anti-Cy5 Antibody (B-2): m-IgG1 BP-HRP Bundle | sc-532567 | 200 µg Ab; 20 µg BP | $361.00 | |||
anti-Cy5 Antibody (B-2) AC | sc-166896 AC | 500 µg/ml, 25% agarose | $424.00 | |||
anti-Cy5 Antibody (B-2) HRP | sc-166896 HRP | 200 µg/ml | $322.00 | |||
anti-Cy5 Antibody (B-2) FITC | sc-166896 FITC | 200 µg/ml | $336.00 | |||
anti-Cy5 Antibody (B-2) PE | sc-166896 PE | 200 µg/ml | $349.00 | |||
anti-Cy5 Antibody (B-2) Alexa Fluor® 488 | sc-166896 AF488 | 200 µg/ml | $364.00 | |||
anti-Cy5 Antibody (B-2) Alexa Fluor® 546 | sc-166896 AF546 | 200 µg/ml | $364.00 | |||
anti-Cy5 Antibody (B-2) Alexa Fluor® 594 | sc-166896 AF594 | 200 µg/ml | $364.00 | |||
anti-Cy5 Antibody (B-2) Alexa Fluor® 647 | sc-166896 AF647 | 200 µg/ml | $364.00 | |||
anti-Cy5 Antibody (B-2) Alexa Fluor® 680 | sc-166896 AF680 | 200 µg/ml | $364.00 | |||
anti-Cy5 Antibody (B-2) Alexa Fluor® 790 | sc-166896 AF790 | 200 µg/ml | $364.00 |