Annexin VII Double Nickase Plasmid (h): sc-402823-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Annexin VII Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Annexin VII Double Nickase Plasmid (h) and Annexin VII Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ANXA7. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Annexin VII Antibody (A-1): sc-17815
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Annexin VII Double Nickase Plasmid (h) | sc-402823-NIC | 20 µg | $410.00 | |||
Annexin VII Double Nickase Plasmid (h2) | sc-402823-NIC-2 | 20 µg | $410.00 |
ANXA7 encodes annexin VII, a Ca²⁺-dependent phospholipid-binding protein that associates with cellular membranes to coordinate membrane trafficking, exocytosis, and membrane repair. Annexin VII contributes to Ca²⁺-regulated vesicle fusion dynamics and has been linked to cytoskeletal remodeling and signal transduction events that depend on lipid microdomain organization. Through its roles at endomembranes and the plasma membrane, ANXA7 can influence stress responses and cellular homeostasis. Altered ANXA7 expression or regulation has been reported in contexts including cancer biology and neurological disease research, supporting investigation of its function in proliferation, migration, and Ca²⁺-dependent secretion pathways.
Annexin VII Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ANXA7 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ANXA7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ANXA7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ANXA7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.