Annexin VI Double Nickase Plasmid (h): sc-401791-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Annexin VI Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Annexin VI Double Nickase Plasmid (h) and Annexin VI Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ANXA6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Annexin VI Antibody (E-5): sc-271859
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Annexin VI Double Nickase Plasmid (h) | sc-401791-NIC | 20 µg | $410.00 | |||
Annexin VI Double Nickase Plasmid (h2) | sc-401791-NIC-2 | 20 µg | $410.00 |
Human ANXA6 encodes annexin VI, a Ca2+-dependent phospholipid-binding scaffold that associates with the inner leaflet of cellular membranes to coordinate membrane trafficking, exocytosis/endocytosis, and cytoskeletal dynamics. Annexin VI participates in calcium signaling and membrane microdomain organization, influencing receptor turnover and signal transduction pathways that depend on regulated vesicle transport. It has been linked to processes such as cholesterol homeostasis and cell migration through its roles in endosomal sorting and membrane–cytoskeleton coupling. Altered ANXA6 expression or localization has been reported in contexts including cancer biology and cardiovascular-related cellular phenotypes, making it relevant for mechanistic studies of membrane-dependent signaling networks.
Annexin VI Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ANXA6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ANXA6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ANXA6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ANXA6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.