Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Annexin V CRISPR/Cas9 KO Plasmid (m): sc-419117
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Annexin V CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Annexin V genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Annexin V Antibody (H-3): sc-74438
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Annexin V CRISPR/Cas9 KO Plasmid (m) | sc-419117 | 20 µg | $397.00 |
Overview
Mouse Anxa5 encodes annexin V, a Ca²⁺-dependent phospholipid-binding protein that associates with the inner leaflet of cellular membranes and is rapidly recruited to sites of membrane remodeling. Annexin V contributes to membrane repair, vesicle trafficking, and regulation of cytoskeletal dynamics, and is widely used as a molecular marker for phosphatidylserine exposure during apoptosis. Through its interactions with anionic phospholipids, annexin V influences calcium signaling–linked processes and can modulate inflammatory and coagulation-relevant membrane events. Altered ANXA5 expression or localization has been associated with dysregulated cell death responses and tissue injury contexts, supporting its study in models of immune activation, cardiovascular biology, and cancer cell stress.
Annexin V CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Anxa5 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Anxa5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Anxa5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Annexin V protein expression.
This CRISPR knockout system enables efficient generation of Anxa5-deficient cell models for investigation of Annexin V signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Anxa5 exon(s) critical for Annexin V function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Anxa5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Annexin V CRISPR/Cas9 KO Plasmid (m) and Annexin V CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Anxa5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Annexin V HDR Plasmid (m) and Annexin V HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Anxa5 homology arms to support homology-directed repair at defined Anxa5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.