Annexin III Double Nickase Plasmid (h): sc-403572-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Annexin III Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Annexin III Double Nickase Plasmid (h) and Annexin III Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ANXA3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Annexin III Antibody (G-1): sc-390700
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Annexin III Double Nickase Plasmid (h) | sc-403572-NIC | 20 µg | $410.00 | |||
Annexin III Double Nickase Plasmid (h2) | sc-403572-NIC-2 | 20 µg | $410.00 |
ANXA3 encodes Annexin III, a Ca²⁺-dependent phospholipid-binding protein that associates with cellular membranes to regulate vesicle trafficking, membrane organization, and cytoskeleton-linked processes. Annexin III has been implicated in signaling networks connected to inflammatory responses and oxidative stress, and it can influence cellular proliferation, migration, and survival through context-dependent modulation of membrane-proximal signaling. Altered ANXA3 expression has been reported across multiple cancer and immune-related research settings, where it is studied as a contributor to tumor biology, chemoresistance-associated phenotypes, and myeloid cell function. These features make ANXA3 a useful target for dissecting membrane dynamics and signaling pathways relevant to disease mechanisms in human cell models.
Annexin III Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ANXA3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ANXA3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ANXA3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ANXA3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.