Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Annexin A10 CRISPR/Cas9 KO Plasmid (h): sc-406977
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Annexin A10 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Annexin A10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Annexin A10 Antibody (E-11): sc-398736
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Annexin A10 CRISPR/Cas9 KO Plasmid (h) | sc-406977 | 20 µg | $397.00 |
Overview
ANXA10 encodes annexin A10, a member of the annexin family of Ca²⁺- and phospholipid-binding proteins implicated in membrane-associated processes. Although annexin A10 lacks some canonical Ca²⁺-binding features found in other annexins, it has been linked to regulation of epithelial cell differentiation, membrane trafficking, and cellular polarity programs that influence tissue homeostasis. ANXA10 expression is enriched in specific epithelial contexts and has been associated with transcriptional states characteristic of mucosal and glandular lineages. Dysregulated ANXA10 expression has been reported in multiple cancer types and is frequently studied as a marker of altered differentiation and tumor subtype biology.
Annexin A10 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ANXA10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ANXA10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ANXA10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Annexin A10 protein expression.
This CRISPR knockout system enables efficient generation of ANXA10-deficient cell models for investigation of Annexin A10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ANXA10 exon(s) critical for Annexin A10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ANXA10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Annexin A10 CRISPR/Cas9 KO Plasmid (h) and Annexin A10 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ANXA10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Annexin A10 HDR Plasmid (h) and Annexin A10 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ANXA10 homology arms to support homology-directed repair at defined ANXA10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.