Date published: 2026-3-27

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ANKZF1 Antibody (B-12): sc-398713

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Datasheets
  • ANKZF1 Antibody (B-12) is a mouse monoclonal IgG2b κ ANKZF1 antibody, cited in 3 publications, provided at 200 µg/ml
  • raised against amino acids 68-355 mapping within an internal region of ANKZF1 of human origin
  • ANKZF1 Antibody (B-12) is recommended for detection of ANKZF1 of human origin by WB, IP, IF and ELISA
  • Anti-ANKZF1 Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG2b BP-HRP is the preferred secondary detection reagent for ANKZF1 Antibody (B-12) for WB applications. This reagent is now offered in a bundle with ANKZF1 Antibody (B-12) (see ordering information below).
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ANKZF1 Antibody (B-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects ANKZF1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ANKZF1 antibody (B-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ANKZF1, also known as ZNF744, is composed of 726 amino acids and is notable for its unique structural features, including one C2H2-type zinc finger and two ankyrin repeats, while lacking a KRAB domain. This structural composition is significant as ankyrin repeats mediate protein-protein interactions, which are crucial for various cellular processes, including signal transduction and transcriptional regulation. ANKZF1 is located on chromosome 2, and ANKZF1 expression is implicated in several biological pathways, making ANKZF1 an important target for research in gene regulation and cellular function.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

ANKZF1 Antibody (B-12) References:

  1. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.  |  Strausberg, RL., et al. 2002. Proc Natl Acad Sci U S A. 99: 16899-903. PMID: 12477932
  2. A human protein-protein interaction network: a resource for annotating the proteome.  |  Stelzl, U., et al. 2005. Cell. 122: 957-68. PMID: 16169070
  3. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes.  |  Kimura, K., et al. 2006. Genome Res. 16: 55-65. PMID: 16344560
  4. A probability-based approach for high-throughput protein phosphorylation analysis and site localization.  |  Beausoleil, SA., et al. 2006. Nat Biotechnol. 24: 1285-92. PMID: 16964243
  5. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.  |  Olsen, JV., et al. 2006. Cell. 127: 635-48. PMID: 17081983
  6. Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.  |  Maruyama, K. and Sugano, S. 1994. Gene. 138: 171-4. PMID: 8125298
  7. Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.  |  Suzuki, Y., et al. 1997. Gene. 200: 149-56. PMID: 9373149

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

ANKZF1 Antibody (B-12)

sc-398713
200 µg/ml
$322.00

ANKZF1 Antibody (B-12): m-IgG2b BP-HRP Bundle

sc-548791
200 µg Ab; 10 µg BP
$361.00

ANKZF1 Antibody (B-12) AC

sc-398713 AC
500 µg/ml, 25% agarose
$424.00

ANKZF1 Antibody (B-12) HRP

sc-398713 HRP
200 µg/ml
$322.00

ANKZF1 Antibody (B-12) FITC

sc-398713 FITC
200 µg/ml
$336.00

ANKZF1 Antibody (B-12) PE

sc-398713 PE
200 µg/ml
$349.00

ANKZF1 Antibody (B-12) Alexa Fluor® 488

sc-398713 AF488
200 µg/ml
$364.00

ANKZF1 Antibody (B-12) Alexa Fluor® 546

sc-398713 AF546
200 µg/ml
$364.00

ANKZF1 Antibody (B-12) Alexa Fluor® 594

sc-398713 AF594
200 µg/ml
$364.00

ANKZF1 Antibody (B-12) Alexa Fluor® 647

sc-398713 AF647
200 µg/ml
$364.00

ANKZF1 Antibody (B-12) Alexa Fluor® 680

sc-398713 AF680
200 µg/ml
$364.00

ANKZF1 Antibody (B-12) Alexa Fluor® 790

sc-398713 AF790
200 µg/ml
$364.00

What is the recommended starting dilution for Western blot for sc-398713: ANKZF1 (B-12) monoclonal antibody?

Asked by: Dr Ninau Qelp
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Great signal in various sample typesI bought this ab and tested it in various cell lines and works really well. Litle background and great sensitivity.
Date published: 2019-03-29
Rated 5 out of 5 by from Very good Western blot data of ANKZF1 expressionVery good Western blot data of ANKZF1 expression in HeLa, SW480, Raji, PANC-1 and RT-4 whole cell lysates. -SCBT QC
Date published: 2014-11-07
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ANKZF1 Antibody (B-12) is rated 5.0 out of 5 by 2.
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