Ankyrin-1 CRISPR/Cas9 KO Plasmid (m): sc-419108
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ankyrin-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ankyrin-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ankyrin-1 Antibody (8C3): sc-12733
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ankyrin-1 CRISPR/Cas9 KO Plasmid (m) | sc-419108 | 20 µg | $397.00 |
Overview
Ank1 encodes ankyrin-1, a membrane adaptor that links integral membrane proteins to the underlying spectrin–actin cytoskeleton, supporting cellular mechanical stability and membrane domain organization. In mouse erythroid cells, ankyrin-1 is a key component of the red blood cell membrane skeleton, coordinating interactions among band 3, spectrin, and associated complexes that influence membrane deformability and ion homeostasis. Disruption of Ank1 perturbs cytoskeletal anchoring and can alter red cell morphology, providing a tractable model for studying hemolytic anemia–like phenotypes and broader principles of membrane–cytoskeleton coupling. Ankyrin-1 function is also relevant to investigations of protein trafficking and compartmentalized signaling at the plasma membrane.
Ankyrin-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ank1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ank1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ank1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ankyrin-1 protein expression.
This CRISPR knockout system enables efficient generation of Ank1-deficient cell models for investigation of Ankyrin-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ank1 exon(s) critical for Ankyrin-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ank1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ankyrin-1 CRISPR/Cas9 KO Plasmid (m) and Ankyrin-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ank1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ankyrin-1 HDR Plasmid (m) and Ankyrin-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ank1 homology arms to support homology-directed repair at defined Ank1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.