Ankyrin-1 Antibody (8C3): sc-12733

Ankyrin-1 Antibody (8C3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ankyrin-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Ankyrin-1 antibody (8C3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ankyrin-1 plays a crucial role in maintaining cellular structural integrity by linking integral membrane proteins to the cytoskeleton, which is essential for cellular stability and function. Ankyrin-1 is predominantly located in the plasma membrane of erythrocytes, cardiac and skeletal muscle, and neurons, where Ankyrin-1 facilitates the organization of membrane domains and anchoring of ion channels and transporters. This localization is vital for processes such as signal transduction and maintaining red blood cell shape, which is particularly important in preventing conditions like hereditary spherocytosis, a disorder characterized by red blood cell fragility. ANK1 gene encodes Ankyrin-1 in multiple isoforms, including a full-length version and several shorter variants, which may have distinct functional roles in different tissues.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ankyrin-1 Antibody (8C3) References:

1. Physiological roles of axonal ankyrins in survival of premyelinated axons and localization of voltage-gated sodium channels.  |  Bennett, V. and Lambert, S. 1999. J Neurocytol. 28: 303-18. PMID: 10739573
2. Expression of sodium channel SNS/PN3 and ankyrin(G) mRNAs in the trigeminal ganglion after inferior alveolar nerve injury in the rat.  |  Bongenhielm, U., et al. 2000. Exp Neurol. 164: 384-95. PMID: 10915577
3. Transient receptor potential ankyrin 1 (TRPA1) antagonists.  |  Preti, D., et al. 2015. Pharm Pat Anal. 4: 75-94. PMID: 25853468
4. Roles of Transient Receptor Potential Ankyrin 1 in Oxaliplatin-Induced Peripheral Neuropathy.  |  Nakagawa, T. and Kaneko, S. 2017. Biol Pharm Bull. 40: 947-953. PMID: 28674258
5. Architecture of the human erythrocyte ankyrin-1 complex.  |  Vallese, F., et al. 2022. Nat Struct Mol Biol. 29: 706-718. PMID: 35835865
6. Transient Receptor Potential Ankyrin 1 (TRPA1) Methylation and Chronic Pain: A Systematic Review.  |  Celsi, F., et al. 2023. Genes (Basel). 14: PMID: 36833338
7. Transient Receptor Potential Ankyrin 1 (TRPA1) Channel as a Sensor of Oxidative Stress in Cancer Cells.  |  Moccia, F. and Montagna, D. 2023. Cells. 12: PMID: 37174661
8. Ankyrin-1 mutations are a major cause of dominant and recessive hereditary spherocytosis.  |  Eber, SW., et al. 1996. Nat Genet. 13: 214-8. PMID: 8640229
9. Small, membrane-bound, alternatively spliced forms of ankyrin 1 associated with the sarcoplasmic reticulum of mammalian skeletal muscle.  |  Zhou, D., et al. 1997. J Cell Biol. 136: 621-31. PMID: 9024692
10. Structure and organization of the human ankyrin-1 gene. Basis for complexity of pre-mRNA processing.  |  Gallagher, PG., et al. 1997. J Biol Chem. 272: 19220-8. PMID: 9235914
11. An alternate promoter directs expression of a truncated, muscle-specific isoform of the human ankyrin 1 gene.  |  Gallagher, PG. and Forget, BG. 1998. J Biol Chem. 273: 1339-48. PMID: 9430667
12. Restriction of 480/270-kD ankyrin G to axon proximal segments requires multiple ankyrin G-specific domains.  |  Zhang, X. and Bennett, V. 1998. J Cell Biol. 142: 1571-81. PMID: 9744885

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ankyrin-1 Antibody (8C3) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Ankyrin-1 Antibody (8C3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ankyrin-1 Antibody (8C3) Specifications

Alternate Product Name: Ankyrin-1

Product Clone Name: 8C3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Ankyrin-1

Epitope Species: human

Product Reactivity/Detection: Ankyrin-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Ankyrin-1 Antibody (8C3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin