ANKRD28/44/52 Antibody (G-6): sc-393032

ANKRD28/44/52 Antibody (G-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects ANKRD28/44/52 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ANKRD28/44/52 (G-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ANKRD28, also known as Ankyrin repeat domain-containing protein 28, is a crucial nuclear protein composed of 1,086 amino acids and characterized by 27 ankyrin repeats, which are essential for protein-protein interactions. ANKRD28 plays a significant role in cellular signaling and regulation, particularly in cell cycle control and transcriptional regulation. Multiple isoforms of ANKRD28, resulting from alternative splicing, allow for diverse functional roles in various tissues, highlighting ANKRD28′s importance in maintaining cellular homeostasis. Ankyrin repeat domains mediate interactions with other proteins, which is vital for assembling multiprotein complexes that regulate critical cellular processes. Mutations in ankyrin genes, including ANKRD28, can lead to severe genetic disorders, underscoring ANKRD28′s importance in health and disease. ANKRD44 and ANKRD52, the other members of this target family, also contribute to protein phosphatase 6 regulatory functions, emphasizing ANKRD28/44/52 complex relevance in cellular signaling pathways.

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ANKRD28/44/52 Antibody (G-6) References:

1. The Chinese hamster dihydrofolate reductase replication origin beta is active at multiple ectopic chromosomal locations and requires specific DNA sequence elements for activity.  |  Altman, AL. and Fanning, E. 2001. Mol Cell Biol. 21: 1098-110. PMID: 11158297
2. PITK, a PP1 targeting subunit that modulates the phosphorylation of the transcriptional regulator hnRNP K.  |  Kwiek, NC., et al. 2006. Cell Signal. 18: 1769-78. PMID: 16564677
3. Array painting reveals a high frequency of balanced translocations in breast cancer cell lines that break in cancer-relevant genes.  |  Howarth, KD., et al. 2008. Oncogene. 27: 3345-59. PMID: 18084325
4. Protein phosphatase 6 regulatory subunits composed of ankyrin repeat domains.  |  Stefansson, B., et al. 2008. Biochemistry. 47: 1442-51. PMID: 18186651
5. Ankyrin repeat domain 28 (ANKRD28), a novel binding partner of DOCK180, promotes cell migration by regulating focal adhesion formation.  |  Tachibana, M., et al. 2009. Exp Cell Res. 315: 863-76. PMID: 19118547
6. Regulation of focal adhesion and cell migration by ANKRD28-DOCK180 interaction.  |  Kiyokawa, E. and Matsuda, M. 2009. Cell Adh Migr. 3: 281-4. PMID: 19458477
7. Mapping of protein phosphatase-6 association with its SAPS domain regulatory subunit using a model of helical repeats.  |  Guergnon, J., et al. 2009. BMC Biochem. 10: 24. PMID: 19835610
8. Protein phosphatase 6 interacts with the DNA-dependent protein kinase catalytic subunit and dephosphorylates gamma-H2AX.  |  Douglas, P., et al. 2010. Mol Cell Biol. 30: 1368-81. PMID: 20065038

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ANKRD28/44/52 Antibody (G-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-ANKRD28/44/52 Antibody (G-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ANKRD28/44/52 Antibody (G-6) Specifications

Alternate Product Name: ANKRD28/44/52

Product Clone Name: G-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 262-394

Epitope Target: ANKRD44

Epitope Species: human

Product Reactivity/Detection: ANKRD28, ANKRD44 and ANKRD52

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for ANKRD28/44/52 Antibody (G-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred ANKRD52 antibody is ANKRD52 Antibody (A-1): sc-398544.