ANKRD22 CRISPR/Cas9 KO Plasmid (m): sc-424577
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ANKRD22 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ANKRD22 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ANKRD22 CRISPR/Cas9 KO Plasmid (m) | sc-424577 | 20 µg | $397.00 |
Overview
Ankrd22 encodes ANKRD22, an ankyrin repeat–containing protein implicated in protein–protein interactions that can influence cellular signaling and stress-adaptive programs. Expression profiling studies have linked ANKRD22 to metabolic state and tissue-specific regulation, suggesting roles in pathways that coordinate energy balance with growth and survival cues. In mammalian systems, ankyrin repeat proteins frequently interface with ubiquitin-dependent turnover, cytoskeletal organization, and transcriptional control, making Ankrd22 relevant for dissecting context-dependent regulatory networks. Altered ANKRD22 expression has been reported in disease-associated datasets, supporting its use as a molecular node for mechanistic studies of dysregulated metabolism and proliferative signaling in vivo and in cell models.
ANKRD22 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ankrd22 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ankrd22 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ankrd22 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ANKRD22 protein expression.
This CRISPR knockout system enables efficient generation of Ankrd22-deficient cell models for investigation of ANKRD22 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ankrd22 exon(s) critical for ANKRD22 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ankrd22 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ANKRD22 CRISPR/Cas9 KO Plasmid (m) and ANKRD22 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ankrd22 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ANKRD22 HDR Plasmid (m) and ANKRD22 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ankrd22 homology arms to support homology-directed repair at defined Ankrd22 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.