ANKH CRISPR/Cas9 KO Plasmid (m): sc-419107
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ANKH CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ANKH genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ANKH CRISPR/Cas9 KO Plasmid (m) | sc-419107 | 20 µg | $397.00 |
Overview
Mouse Ank encodes ANKH, a multipass membrane protein that regulates the transport of inorganic pyrophosphate (PPi) across the plasma membrane and helps maintain extracellular PPi homeostasis. By modulating the PPi/phosphate balance, ANKH influences biomineralization, matrix mineral deposition, and osteo/chondrocyte-associated processes linked to skeletal development and tissue calcification. ANKH function intersects with pathways controlling mineral metabolism and extracellular matrix remodeling, making it relevant to studies of aberrant calcification and joint or bone phenotypes. Disruption of Ank is therefore used to probe mechanisms that couple PPi handling to cellular differentiation and mineral deposition outcomes in vivo and in cultured mouse cells.
ANKH CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ank gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ank together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ank open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ANKH protein expression.
This CRISPR knockout system enables efficient generation of Ank-deficient cell models for investigation of ANKH signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ank exon(s) critical for ANKH function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ank genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ANKH CRISPR/Cas9 KO Plasmid (m) and ANKH CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ank locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ANKH HDR Plasmid (m) and ANKH HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ank homology arms to support homology-directed repair at defined Ank target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.