Anillin Antibody (C-7): sc-514638

Anillin Antibody (C-7) is a mouse monoclonal IgM antibody that detects Anillin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anillin is an evolutionarily conserved actin-binding protein that plays a crucial role in cytokinesis, a process essential for cell division. Anillin is predominantly localized in the nucleus and is highly expressed in the central nervous system, where Anillin localizes to the cleavage furrow during cytokinesis, forming a contractile ring with the assistance of Rac GTPase. Anti-Anillin antibody (C-7) interacts with key proteins such as CD2AP, which helps concentrate Rho A and maintain the localization of active Myosin, ensuring successful cleavage furrow ingression. In the absence of Anillin, cells experience a failure in completing this critical process, leading to potential cell division errors. Notably, Anillin expression levels fluctuate throughout the cell cycle, peaking during mitosis, and Anillin associates with E-cadherin before the cell transitions into G1, where Anillin undergoes ubiquitination by the anaphase-promoting complex/cyclosome (APC/C). The APC/C recognizes the D-box domain located at the N-terminal region of Anillin, highlighting Anillin′s regulatory mechanisms. Furthermore, Anillin is often overexpressed in various tumors, suggesting Anillin′s potential as a biomarker for cancer diagnosis and prognosis. Anillin monoclonal antibody (C-7) is an invaluable tool for researchers investigating the dynamics of cell division and the implications of Anillin in cancer biology.

The preferred Anillin antibody is Anillin Antibody (B-10): sc-271814.