Angiotensin Receptor/AT1/AGTR1 Double Nickase Plasmid (h): sc-400229-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Angiotensin Receptor/AT1/AGTR1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Angiotensin Receptor/AT1/AGTR1 Double Nickase Plasmid (h) and Angiotensin Receptor/AT1/AGTR1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AGTR1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Angiotensin Receptor/AT1/AGTR1 Antibody (G-3): sc-515884
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Angiotensin Receptor/AT1/AGTR1 Double Nickase Plasmid (h) | sc-400229-NIC | 20 µg | $410.00 | |||
Angiotensin Receptor/AT1/AGTR1 Double Nickase Plasmid (h2) | sc-400229-NIC-2 | 20 µg | $410.00 |
AGTR1 encodes the angiotensin II type 1 receptor (AT1), a seven-transmembrane GPCR that mediates most canonical angiotensin II signaling in cardiovascular and renal tissues. Upon ligand binding, AGTR1 couples predominantly to Gq/11 to activate phospholipase C, calcium mobilization, and PKC signaling, and it can also engage MAPK/ERK, JAK/STAT, NADPH oxidase–dependent ROS, and β-arrestin pathways to regulate transcriptional programs, proliferation, inflammation, and extracellular matrix remodeling. Receptor desensitization and trafficking through GRKs and β-arrestins further shape signal duration and biased signaling outcomes. Dysregulated AGTR1 signaling is implicated in hypertension, cardiac hypertrophy and fibrosis, vascular remodeling, and kidney disease, supporting its use in studies of renin–angiotensin system biology and stress-response pathways.
Angiotensin Receptor/AT1/AGTR1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AGTR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AGTR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AGTR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AGTR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.