Angiomotin Antibody (B-4): sc-166924
- Angiomotin Antibody (B-4) is a mouse monoclonal IgG2b κ Angiomotin antibody, cited in 13 publications, provided at 200 µg/ml
- raised against amino acids 174-279 mapping within an internal region of Angiomotin of human origin
- Angiomotin Antibody (B-4) is recommended for detection of Angiomotin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Angiomotin Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for Angiomotin Antibody (B-4) for WB applications. This reagent is now offered in a bundle with Angiomotin Antibody (B-4) (see ordering information below).
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Angiomotin Antibody (B-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects Angiomotin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Angiomotin antibody (B-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Angiomotin, also known as AMOT, is a significant 1,084 amino acid protein belonging to the motin family of angiostatin-binding proteins, characterized by conserved coiled-coil domains and PDZ binding motifs at their C-termini. Angiomotin is primarily expressed in skeletal muscle and placenta, where Angiomotin localizes to the cell surface at tight junctions, playing a crucial role in maintaining these structures. Angiomotin′s interaction with angiostatin is vital for angiogenesis, as Angiomotin promotes tubule formation and enhances the migration of endothelial cells in response to growth factors. This function underscores Angiomotin′s potential as a key player in tumor angiogenesis, making Angiomotin a promising therapeutic target in cancer treatment. Due to alternative splicing, Angiomotin exists in two isoforms, p130 and p80, with the p130 isoform exhibiting a distinct expression pattern and the ability to interact with F-actin, thereby inducing actin fiber formation, which is essential for various cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Angiomotin Antibody (B-4) References:
- Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. | Kikuno, R., et al. 1999. DNA Res. 6: 197-205. PMID: 10470851
- Angiomotin: an angiostatin binding protein that regulates endothelial cell migration and tube formation. | Troyanovsky, B., et al. 2001. J Cell Biol. 152: 1247-54. PMID: 11257124
- p130-angiomotin associates to actin and controls endothelial cell shape. | Ernkvist, M., et al. 2006. FEBS J. 273: 2000-11. PMID: 16640563
- A Rich1/Amot complex regulates the Cdc42 GTPase and apical-polarity proteins in epithelial cells. | Wells, CD., et al. 2006. Cell. 125: 535-48. PMID: 16678097
- Amotl2 is essential for cell movements in zebrafish embryo and regulates c-Src translocation. | Huang, H., et al. 2007. Development. 134: 979-88. PMID: 17293535
- Angiomotin regulates endothelial cell migration during embryonic angiogenesis. | Aase, K., et al. 2007. Genes Dev. 21: 2055-68. PMID: 17699752
- Therapeutic antibodies targeting angiomotin inhibit angiogenesis in vivo. | Levchenko, T., et al. 2008. FASEB J. 22: 880-9. PMID: 17984175
- Differential roles of p80- and p130-angiomotin in the switch between migration and stabilization of endothelial cells. | Ernkvist, M., et al. 2008. Biochim Biophys Acta. 1783: 429-37. PMID: 18164266
- Angiomotin'g YAP into the nucleus for cell proliferation and cancer development. | Hong, W. 2013. Sci Signal. 6: pe27. PMID: 24003252
- Emerging roles for angiomotin in the nervous system. | Wigerius, M., et al. 2020. Sci Signal. 13: PMID: 33109746
- Proteolytic activation of angiomotin by DDI2 promotes angiogenesis. | Wang, Y., et al. 2023. EMBO J. 42: e112900. PMID: 37350545
- Angiomotin family proteins in the Hippo signaling pathway. | Wang, Y. and Yu, FX. 2024. Bioessays. 46: e2400076. PMID: 38760875
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Angiomotin Antibody (B-4) | sc-166924 | 200 µg/ml | $322.00 | |||
Angiomotin Antibody (B-4): m-IgG2b BP-HRP Bundle | sc-548683 | 200 µg Ab; 10 µg BP | $361.00 | |||
Angiomotin Antibody (B-4) AC | sc-166924 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Angiomotin Antibody (B-4) HRP | sc-166924 HRP | 200 µg/ml | $322.00 | |||
Angiomotin Antibody (B-4) FITC | sc-166924 FITC | 200 µg/ml | $336.00 | |||
Angiomotin Antibody (B-4) PE | sc-166924 PE | 200 µg/ml | $349.00 | |||
Angiomotin Antibody (B-4) Alexa Fluor® 488 | sc-166924 AF488 | 200 µg/ml | $364.00 | |||
Angiomotin Antibody (B-4) Alexa Fluor® 546 | sc-166924 AF546 | 200 µg/ml | $364.00 | |||
Angiomotin Antibody (B-4) Alexa Fluor® 594 | sc-166924 AF594 | 200 µg/ml | $364.00 | |||
Angiomotin Antibody (B-4) Alexa Fluor® 647 | sc-166924 AF647 | 200 µg/ml | $364.00 | |||
Angiomotin Antibody (B-4) Alexa Fluor® 680 | sc-166924 AF680 | 200 µg/ml | $364.00 | |||
Angiomotin Antibody (B-4) Alexa Fluor® 790 | sc-166924 AF790 | 200 µg/ml | $364.00 |