ANG III CRISPR/Cas9 KO Plasmid (m): sc-419105
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ANG III CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ANG III genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ANG III CRISPR/Cas9 KO Plasmid (m) | sc-419105 | 20 µg | $397.00 |
Overview
Mouse Ang3 encodes angiopoietin-3 (ANG III), a secreted ligand of the TEK/TIE2 receptor tyrosine kinase that regulates vascular development and endothelial homeostasis. ANG III participates in angiopoietin–TIE signaling that coordinates endothelial cell survival, vessel maturation, permeability, and remodeling in concert with VEGF-dependent cues. Altered angiopoietin pathway activity is linked to dysregulated angiogenesis and inflammatory vascular responses relevant to studies of tumor vascularization, ischemic injury, and retinovascular disease mechanisms. As a context-dependent modulator of endothelial signaling, Ang3 provides a tractable node for dissecting microenvironmental control of vascular stability and tissue perfusion in mouse models.
ANG III CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ang3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ang3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ang3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ANG III protein expression.
This CRISPR knockout system enables efficient generation of Ang3-deficient cell models for investigation of ANG III signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ang3 exon(s) critical for ANG III function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ang3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ANG III CRISPR/Cas9 KO Plasmid (m) and ANG III CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ang3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ANG III HDR Plasmid (m) and ANG III HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ang3 homology arms to support homology-directed repair at defined Ang3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.