Androgen Receptor Double Nickase Plasmid (h): sc-400026-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Androgen Receptor Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Androgen Receptor Double Nickase Plasmid (h) and Androgen Receptor Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Androgen Receptor Antibody (441): sc-7305
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Androgen Receptor Double Nickase Plasmid (h) | sc-400026-NIC | 20 µg | $410.00 | |||
Androgen Receptor Double Nickase Plasmid (h2) | sc-400026-NIC-2 | 20 µg | $410.00 |
Human AR encodes the androgen receptor, a ligand-activated nuclear hormone receptor that functions as a transcription factor controlling programs involved in differentiation, proliferation, and metabolic homeostasis. Upon binding androgens, AR translocates to the nucleus and engages androgen response elements while coordinating with chromatin remodelers and co-regulators to shape context-dependent gene expression. AR signaling intersects with PI3K/AKT, MAPK, and DNA damage response networks, influencing cell-cycle control and stress adaptation. Dysregulated AR activity and altered receptor variants are implicated in androgen-dependent phenotypes and are extensively studied in prostate cancer biology and endocrine-related disorders.
Androgen Receptor Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.