AMPD1 Antibody (D-7): sc-393117
- AMPD1 Antibody (D-7) is a mouse monoclonal IgG1 λ AMPD1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 104-181 mapping near the N-terminus of AMPD1 of human origin
- AMPD1 Antibody (D-7) is recommended for detection of AMPD1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-AMPD1 Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for AMPD1 Antibody (D-7) for WB applications. This reagent is now offered in a bundle with AMPD1 Antibody (D-7) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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AMPD1 Antibody (D-7) is a mouse monoclonal IgG1 antibody that detects AMPD1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AMPD1 (D-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AMPD1 protein, or adenosine monophosphate deaminase, is a crucial cytosolic enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate (AMP) to inosine monophosphate (IMP) and ammonia, thereby playing a vital role in the purine nucleotide cycle and energy metabolism. Structurally, AMPD1 functions as a homotetramer and is one of three isozymes of AMP deaminase found in mammals, with the others being AMPD2 and AMPD3. While all three isozymes share a conserved C-terminal catalytic domain, they exhibit differences in their N-terminal domains, which may influence their specific regulatory mechanisms and tissue distribution. AMPD1 is predominantly expressed in skeletal muscle, while AMPD2 is found in undifferentiated myoblasts, smooth muscle, embryonic muscle, and non-muscle tissues, and AMPD3 is primarily expressed in erythrocytes. Deficiencies in the AMPD1 gene can lead to adenosine monophosphate deaminase deficiency muscle type (AMPDDM), a metabolic disorder characterized by exercise-related myopathy, which manifests as muscle aches, cramps, and early fatigue during physical activity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
AMPD1 Antibody (D-7) References:
- Localization of N-terminal sequences in human AMP deaminase isoforms that influence contractile protein binding. | Mahnke-Zizelman, DK. and Sabina, RL. 2001. Biochem Biophys Res Commun. 285: 489-95. PMID: 11444869
- Expression, purification, and inhibition of in vitro proteolysis of human AMPD2 (isoform L) recombinant enzymes. | Haas, AL. and Sabina, RL. 2003. Protein Expr Purif. 27: 293-303. PMID: 12597889
- Full-size form of human liver AMP-deaminase? | Szydlowska, M., et al. 2004. Mol Cell Biochem. 266: 133-7. PMID: 15646034
- Clinical significance and neuropathology of primary MADD in C34-T and G468-T mutations of the AMPD1 gene. | Fischer, S., et al. 2005. Clin Neuropathol. 24: 77-85. PMID: 15803807
- Expression patterns of AMP-deaminase and cytosolic 5'-nucleotidase genes in human term placenta. | Roszkowska, A., et al. 2008. Mol Cell Biochem. 311: 249-51. PMID: 18165923
- [AMPD genes and urate metabolism]. | Morisaki, H. and Morisaki, T. 2008. Nihon Rinsho. 66: 771-7. PMID: 18409530
- Cloning, sequence and characterization of the human AMPD2 gene: evidence for transcriptional regulation by two closely spaced promoters. | Mahnke-Zizelman, DK., et al. 1996. Biochim Biophys Acta. 1308: 122-32. PMID: 8764830
- Regulation of rat AMP deaminase 3 (isoform C) by development and skeletal muscle fibre type. | Mahnke-Zizelman, DK., et al. 1997. Biochem J. 326 (Pt 2): 521-9. PMID: 9291127
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AMPD1 Antibody (D-7) | sc-393117 | 200 µg/ml | $316.00 | |||
AMPD1 Antibody (D-7): m-IgGλ BP-HRP Bundle | sc-523811 | 200 µg Ab, 40 µg BP | $354.00 | |||
AMPD1 Antibody (D-7) AC | sc-393117 AC | 500 µg/ml, 25% agarose | $416.00 | |||
AMPD1 Antibody (D-7) HRP | sc-393117 HRP | 200 µg/ml | $316.00 | |||
AMPD1 Antibody (D-7) FITC | sc-393117 FITC | 200 µg/ml | $330.00 | |||
AMPD1 Antibody (D-7) PE | sc-393117 PE | 200 µg/ml | $343.00 | |||
AMPD1 Antibody (D-7) Alexa Fluor® 488 | sc-393117 AF488 | 200 µg/ml | $357.00 | |||
AMPD1 Antibody (D-7) Alexa Fluor® 546 | sc-393117 AF546 | 200 µg/ml | $357.00 | |||
AMPD1 Antibody (D-7) Alexa Fluor® 594 | sc-393117 AF594 | 200 µg/ml | $357.00 | |||
AMPD1 Antibody (D-7) Alexa Fluor® 647 | sc-393117 AF647 | 200 µg/ml | $357.00 | |||
AMPD1 Antibody (D-7) Alexa Fluor® 680 | sc-393117 AF680 | 200 µg/ml | $357.00 | |||
AMPD1 Antibody (D-7) Alexa Fluor® 790 | sc-393117 AF790 | 200 µg/ml | $357.00 |