AMBP Double Nickase Plasmid (h): sc-403136-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AMBP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- AMBP Double Nickase Plasmid (h) and AMBP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AMBP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AMBP Antibody (532): sc-81948
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AMBP Double Nickase Plasmid (h) | sc-403136-NIC | 20 µg | $410.00 | |||
AMBP Double Nickase Plasmid (h2) | sc-403136-NIC-2 | 20 µg | $410.00 |
AMBP encodes the alpha-1-microglobulin/bikunin precursor, a secreted glycoprotein that is proteolytically processed into alpha-1-microglobulin and bikunin, which circulate in plasma and are present in extracellular fluids. Alpha-1-microglobulin functions as a heme- and radical-binding scavenger implicated in redox homeostasis and protection from oxidative stress, while bikunin is a Kunitz-type serine protease inhibitor that contributes to extracellular protease regulation and inflammatory modulation. Through bikunin-containing inter-alpha-inhibitor complexes, AMBP is linked to extracellular matrix stabilization, coagulation-related protease networks, and tissue injury responses. Altered AMBP expression and processing have been associated with inflammatory states, renal and hepatic dysfunction markers, and remodeling processes relevant to diverse disease-model contexts.
AMBP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AMBP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AMBP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AMBP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AMBP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.