AMBP CRISPR/Cas9 KO Plasmid (m): sc-419097
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AMBP CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AMBP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AMBP CRISPR/Cas9 KO Plasmid (m) | sc-419097 | 20 µg | $397.00 |
Overview
Ambp encodes the AMBP precursor protein, which is proteolytically processed to yield alpha-1-microglobulin and inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4). Alpha-1-microglobulin functions as a heme-binding, redox-active scavenger that helps limit oxidative and carbonyl stress, while ITIH4 is associated with extracellular matrix dynamics and acute-phase inflammatory responses. In mouse biology, AMBP-derived products are commonly studied in the context of systemic stress signaling, liver-enriched secreted protein networks, and protease/inflammation-linked remodeling. Dysregulation of these pathways is relevant to research on inflammation, tissue injury, and oxidative stress-associated phenotypes without implying clinical outcomes.
AMBP CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ambp gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ambp together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ambp open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AMBP protein expression.
This CRISPR knockout system enables efficient generation of Ambp-deficient cell models for investigation of AMBP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ambp exon(s) critical for AMBP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ambp genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AMBP CRISPR/Cas9 KO Plasmid (m) and AMBP CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ambp locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AMBP HDR Plasmid (m) and AMBP HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ambp homology arms to support homology-directed repair at defined Ambp target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.