AMAP-1 Antibody (E-5): sc-377495
- AMAP-1 Antibody (E-5) is a mouse monoclonal IgG1 κ AMAP-1 antibody provided at 200 µg/ml
- raised against amino acids 648-947 mapping at the C-terminus of AMAP-1 of human origin
- AMAP-1 Antibody (E-5) is recommended for detection of AMAP-1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-AMAP-1 Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of AMAP-1 (E-5): sc-377495.
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for AMAP-1 Antibody (E-5) for WB applications. This reagent is now offered in a bundle with AMAP-1 Antibody (E-5) (see ordering information below).
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SEE ALSO...
AMAP-1 Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects AMAP-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AMAP-1 (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AMAP-1, also known as AMY-1-binding protein 1, AMAM-1, or MYCBPAP (MYCBP associated protein), consists of 947 amino acids and is predominantly expressed in the testis, where AMAP-1 plays a crucial role in spermatogenesis and synaptic processes. AMAP-1 interacts with MYCBP (AMY-1) and is localized in the cytoplasm as well as at the membrane, highlighting AMAP-1′s importance in cellular signaling pathways. The gene encoding AMAP-1 is located on human chromosome 17, a region comprising over 2.5% of the human genome and encoding more than 1,200 genes, with significant association to tumor suppressor genes such as p53 and BRCA1. p53 is essential for maintaining cellular genetic integrity by regulating cell fate decisions between DNA repair and apoptosis, while BRCA1 is recognized for DNA repair functions and association with hereditary breast and ovarian cancers. AMAP-1′s interactions may provide insights into molecular mechanisms underlying these critical biological processes and their implications in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
AMAP-1 Antibody (E-5) References:
- Molecular cloning and functional expression of two members of mouse NeuAcalpha2,3Galbeta1,3GalNAc GalNAcalpha2,6-sialyltransferase family, ST6GalNAc III and IV. | Lee, YC., et al. 1999. J Biol Chem. 274: 11958-67. PMID: 10207017
- Cloning and expression of a human gene encoding an N-acetylgalactosamine-alpha2,6-sialyltransferase (ST6GalNAc I): a candidate for synthesis of cancer-associated sialyl-Tn antigens. | Ikehara, Y., et al. 1999. Glycobiology. 9: 1213-24. PMID: 10536037
- AMAP-1, a novel testis-specific AMY-1-binding protein, is differentially expressed during the course of spermatogenesis. | Yukitake, H., et al. 2002. Biochim Biophys Acta. 1577: 126-32. PMID: 12151104
- Recognition of cell surface acceptors by two human alpha-2,6-sialyltransferases produced in CHO cells. | Donadio, S., et al. 2003. Biochimie. 85: 311-21. PMID: 12770770
- Expression of sialyl-Tn antigen in breast cancer cells transfected with the human CMP-Neu5Ac: GalNAc alpha2,6-sialyltransferase (ST6GalNac I) cDNA. | Julien, S., et al. 2001. Glycoconj J. 18: 883-93. PMID: 12820722
- The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer. | Sewell, R., et al. 2006. J Biol Chem. 281: 3586-94. PMID: 16319059
- IRES-mediated translational control of AMAP1 expression during differentiation of monocyte U937 cells. | Miyata, M., et al. 2008. Cell Cycle. 7: 3273-81. PMID: 18843202
- Prognostic utility of glycosyltransferase expression in breast cancer. | Patani, N., et al. 2008. Cancer Genomics Proteomics. 5: 333-40. PMID: 19287074
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AMAP-1 Antibody (E-5) | sc-377495 | 200 µg/ml | $316.00 | |||
AMAP-1 Antibody (E-5): m-IgG Fc BP-HRP Bundle | sc-526104 | 200 µg Ab; 10 µg BP | $354.00 | |||
AMAP-1 Antibody (E-5) AC | sc-377495 AC | 500 µg/ml, 25% agarose | $416.00 | |||
AMAP-1 Antibody (E-5) HRP | sc-377495 HRP | 200 µg/ml | $316.00 | |||
AMAP-1 Antibody (E-5) FITC | sc-377495 FITC | 200 µg/ml | $330.00 | |||
AMAP-1 Antibody (E-5) PE | sc-377495 PE | 200 µg/ml | $343.00 | |||
AMAP-1 Antibody (E-5) Alexa Fluor® 488 | sc-377495 AF488 | 200 µg/ml | $357.00 | |||
AMAP-1 Antibody (E-5) Alexa Fluor® 546 | sc-377495 AF546 | 200 µg/ml | $357.00 | |||
AMAP-1 Antibody (E-5) Alexa Fluor® 594 | sc-377495 AF594 | 200 µg/ml | $357.00 | |||
AMAP-1 Antibody (E-5) Alexa Fluor® 647 | sc-377495 AF647 | 200 µg/ml | $357.00 | |||
AMAP-1 Antibody (E-5) Alexa Fluor® 680 | sc-377495 AF680 | 200 µg/ml | $357.00 | |||
AMAP-1 Antibody (E-5) Alexa Fluor® 790 | sc-377495 AF790 | 200 µg/ml | $357.00 |