ALPPL2 Antibody (9-YD35): sc-134255

ALPPL2 Antibody (9-YD35) is a mouse monoclonal IgG2a kappa light chain antibody that detects the ALPPL2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-ALPPL2 antibody (9-YD35) is available as a non-conjugated format. ALPPL2, also known as alkaline phosphatase, placental-like 2, ALPG, or GCAP, is a 532 amino acid protein that is lipid-anchored to the cell membrane and belongs to the alkaline phosphatase family. ALPPL2 plays a crucial role in dephosphorylating various substrates, which is essential for numerous biological processes, including cellular signaling and metabolism. ALPPL2 is predominantly expressed in the thymus and testis, and functions as a homodimer that utilizes magnesium and zinc as cofactors to catalyze the conversion of a phosphate monoester into an alcohol and a free phosphate. ALPPL2 is overexpressed in germ cell tumors, indicating potential involvement in tumor development and progression. The gene encoding ALPPL2 is located on human chromosome 2, which contains over 1,400 genes and accounts for nearly 8% of the human genome. Understanding ALPPL2 function is vital, as research may provide insights into the mechanisms underlying certain cancers and could potentially serve as a therapeutic target.

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ALPPL2 Antibody (9-YD35) References:

1. Structural evidence of functional divergence in human alkaline phosphatases.  |  Le Du, MH. and Millan, JL. 2002. J Biol Chem. 277: 49808-14. PMID: 12372831
2. Mouse models to study GCAP functions in intact photoreceptors.  |  Mendez, A. and Chen, J. 2002. Adv Exp Med Biol. 514: 361-88. PMID: 12596933
3. Site-directed mutagenesis and epitope-mapped monoclonal antibodies define a catalytically important conformational difference between human placental and germ cell alkaline phosphatase.  |  Hoylaerts, MF. and Millán, JL. 1991. Eur J Biochem. 202: 605-16. PMID: 1722150
4. The expression of placental alkaline phosphatase (PLAP) and PLAP-like enzymes in normal and neoplastic human tissues. An immunohistological survey using monoclonal antibodies.  |  Hamilton-Dutoit, SJ., et al. 1990. APMIS. 98: 797-811. PMID: 2171580
5. Characterization of the placental alkaline phosphatase-like (Nagao) isozyme on the surface of A431 human epidermoid carcinoma cells.  |  Jemmerson, R., et al. 1985. Cancer Res. 45: 282-7. PMID: 2578098
6. ALPPL2 Aptamer-Mediated Targeted Delivery of 5-Fluoro-2'-Deoxyuridine to Pancreatic Cancer.  |  Dua, P., et al. 2015. Nucleic Acid Ther. 25: 180-7. PMID: 25919296
7. High expression of ALPPL2 is associated with poor prognosis in gastric cancer.  |  Liu, S., et al. 2019. Hum Pathol. 86: 49-56. PMID: 30496798
8. ALPPL2 Is a Highly Specific and Targetable Tumor Cell Surface Antigen.  |  Su, Y., et al. 2020. Cancer Res. 80: 4552-4564. PMID: 32868383
9. 5' nucleotide sequence of a putative human placental alkaline phosphatase-like gene.  |  Shen, LP., et al. 1988. Nucleic Acids Res. 16: 5694. PMID: 3387245
10. Molecular cloning and sequence analysis of human placental alkaline phosphatase.  |  Millán, JL. 1986. J Biol Chem. 261: 3112-5. PMID: 3512548
11. The human placental alkaline phosphatase gene and related sequences map to chromosome 2 band q37.  |  Martin, D., et al. 1987. Ann Hum Genet. 51: 145-52. PMID: 3674755
12. A possible new locus of alkaline phosphatase expressed in human testis.  |  Millán, JL., et al. 1982. Hum Genet. 62: 293-5. PMID: 7166303

The preferred TNAP antibody is TNAP Antibody (F-4): sc-166261.