αPIX Antibody (2E5): sc-293362

αPIX Antibody (2E5) is a mouse monoclonal IgG2a kappa light chain antibody that detects αPIX protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-αPIX antibody (2E5) is available as the non-conjugated form. αPIX protein plays a crucial role in cellular signaling as a guanine nucleotide exchange factor (GEF) for Rac, a member of the Rho family of GTPases, which is essential for regulating actin cytoskeleton dynamics and cell motility. This function is particularly important in processes such as lamellipodia formation, which are critical for cell migration and tissue development. The interaction of αPIX with p21 activated kinase (PAK) is vital for the localization and activation of PAK in the Cdc42 to Rac signaling pathway, highlighting the intricate network of protein interactions that govern cellular responses to external stimuli. Additionally, αPIX exists in two isoforms, α and β, both of which are highly expressed in human and rat heart, muscle, and thymus tissues, underscoring their significance in various physiological processes. The phosphorylation of αPIX through pathways such as platelet-derived growth factor (PDGF) and EphB2 receptor signaling further modulates its activity, making αPIX a key player in cellular signaling cascades.

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αPIX Antibody (2E5) References:

1. alphaPix stimulates p21-activated kinase activity through exchange factor-dependent and -independent mechanisms.  |  Daniels, RH., et al. 1999. J Biol Chem. 274: 6047-50. PMID: 10037684
2. Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling.  |  Turner, CE., et al. 1999. J Cell Biol. 145: 851-63. PMID: 10330411
3. alphaPIX nucleotide exchange factor is activated by interaction with phosphatidylinositol 3-kinase.  |  Yoshii, S., et al. 1999. Oncogene. 18: 5680-90. PMID: 10523848
4. Directional sensing requires G beta gamma-mediated PAK1 and PIX alpha-dependent activation of Cdc42.  |  Li, Z., et al. 2003. Cell. 114: 215-27. PMID: 12887923
5. Novel regulatory mechanisms for the Dbl family guanine nucleotide exchange factor Cool-2/alpha-Pix.  |  Feng, Q., et al. 2004. EMBO J. 23: 3492-504. PMID: 15306850
6. The Cool-2/alpha-Pix protein mediates a Cdc42-Rac signaling cascade.  |  Baird, D., et al. 2005. Curr Biol. 15: 1-10. PMID: 15649357
7. PAK kinases are directly coupled to the PIX family of nucleotide exchange factors.  |  Manser, E., et al. 1998. Mol Cell. 1: 183-92. PMID: 9659915
8. PAK promotes morphological changes by acting upstream of Rac.  |  Obermeier, A., et al. 1998. EMBO J. 17: 4328-39. PMID: 9687501