αC-crystallin Antibody (H-5): sc-398395
- αC-crystallin Antibody (H-5) is a mouse monoclonal IgG1 κ αC-crystallin antibody provided at 200 µg/ml
- raised against amino acids 24-88 mapping within an internal region of αC-crystallin of human origin
- alpha C-crystallin Antibody (H-5) is recommended for detection of αC-crystallin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-alpha C-crystallin Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for αC-crystallin Antibody (H-5) for WB applications. These reagents are now offered in bundles with αC-crystallin Antibody (H-5) (see ordering information below).
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αC-crystallin Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects αC-crystallin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-αC-crystallin antibody (H-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. αC-crystallin plays a crucial role in maintaining lens transparency and refractive index, which is vital for proper vision. Located primarily in the eye lens, crystallins, including αC-crystallin, are essential for structural integrity of the lens, preventing light scattering that can lead to cataract formation. Structurally, αC-crystallin belongs to the small heat shock protein family and consists of three gene products: αA, αB, and αC. These proteins feature a unique seven-domain structure, which includes four homologous motifs and a connecting peptide, allowing effective function as molecular chaperones. While αC-crystallin does not renature denatured proteins, this molecular chaperone stabilizes proteins in large soluble aggregates, thus preventing aggregation and maintaining cellular homeostasis. Research has shown that αC-crystallin interacts with membrane proteins, specifically at the polar-apolar interface, influencing lipid bilayer mobility and highlighting significance in cellular function and integrity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
αC-crystallin Antibody (H-5) References:
- Mutation R120G in alphaB-crystallin, which is linked to a desmin-related myopathy, results in an irregular structure and defective chaperone-like function. | Bova, MP., et al. 1999. Proc Natl Acad Sci U S A. 96: 6137-42. PMID: 10339554
- alpha-crystallin prevents irreversible protein denaturation and acts cooperatively with other heat-shock proteins to renature the stabilized partially denatured protein in an ATP-dependent manner. | Wang, K. and Spector, A. 2000. Eur J Biochem. 267: 4705-12. PMID: 10903503
- Lens crystallins and their microbial homologs: structure, stability, and function. | Jaenicke, R. and Slingsby, C. 2001. Crit Rev Biochem Mol Biol. 36: 435-99. PMID: 11724156
- Alpha-crystallin-type heat shock proteins: socializing minichaperones in the context of a multichaperone network. | Narberhaus, F. 2002. Microbiol Mol Biol Rev. 66: 64-93; table of contents. PMID: 11875128
- Differential expression of B-crystallin and Hsp27 in skeletal muscle during continuous contractile activity. Relationship to myogenic regulatory factors. | Neufer, PD. and Benjamin, IJ. 1996. J Biol Chem. 271: 24089-95. PMID: 8798647
- Autosomal dominant congenital cataract associated with a missense mutation in the human alpha crystallin gene CRYAA. | Litt, M., et al. 1998. Hum Mol Genet. 7: 471-4. PMID: 9467006
- The small heat-shock protein, alphaB-crystallin, has a variable quaternary structure. | Haley, DA., et al. 1998. J Mol Biol. 277: 27-35. PMID: 9514758
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
αC-crystallin Antibody (H-5) | sc-398395 | 200 µg/ml | $322.00 | |||
αC-crystallin Antibody (H-5): m-IgG Fc BP-HRP Bundle | sc-527770 | 200 µg Ab; 10 µg BP | $361.00 | |||
αC-crystallin Antibody (H-5): m-IgG1 BP-HRP Bundle | sc-533143 | 200 µg Ab; 20 µg BP | $361.00 | |||
αC-crystallin Antibody (H-5) AC | sc-398395 AC | 500 µg/ml, 25% agarose | $424.00 | |||
αC-crystallin Antibody (H-5) HRP | sc-398395 HRP | 200 µg/ml | $322.00 | |||
αC-crystallin Antibody (H-5) FITC | sc-398395 FITC | 200 µg/ml | $336.00 | |||
αC-crystallin Antibody (H-5) PE | sc-398395 PE | 200 µg/ml | $349.00 | |||
αC-crystallin Antibody (H-5) Alexa Fluor® 488 | sc-398395 AF488 | 200 µg/ml | $364.00 | |||
αC-crystallin Antibody (H-5) Alexa Fluor® 546 | sc-398395 AF546 | 200 µg/ml | $364.00 | |||
αC-crystallin Antibody (H-5) Alexa Fluor® 594 | sc-398395 AF594 | 200 µg/ml | $364.00 | |||
αC-crystallin Antibody (H-5) Alexa Fluor® 647 | sc-398395 AF647 | 200 µg/ml | $364.00 | |||
αC-crystallin Antibody (H-5) Alexa Fluor® 680 | sc-398395 AF680 | 200 µg/ml | $364.00 | |||
αC-crystallin Antibody (H-5) Alexa Fluor® 790 | sc-398395 AF790 | 200 µg/ml | $364.00 |