Anti-alpha 3 Tubulin Antibody (10J1) recommended for detection of α3 Tubulin and α1 Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-α3 Tubulin Antibody (10J1): sc-134240

Anti-α3 Tubulin Antibody (10J1) is rated 4.0 out of 5 by 3.
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Datasheets

    • Anti-α3 Tubulin Antibody (10J1) is a mouse monoclonal IgG1 κ α3 Tubulin antibody, cited in 3 publications, provided at 100 µg/ml
    • raised against recombinant α3C Tubulin protein of human origin
    • recommended for detection of α3 Tubulin and α1 Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α3 Tubulin (10J1): sc-134240.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for α3 Tubulin Antibody (10J1). This work is in progress.
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Can sc-134240: å3 Tubulin (10J1) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, sc-134240: å3 Tubulin (10J1) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from GOOD antibody I bought this antibody two months age, and I am very glad that this antibody is very efficient. I uesd this Antibody of 1:1000 dilution in Wb, second antibody is 1:8000. loaded ~30 ug. The band is clearly.
Date published: 2016-12-07
Rated 3 out of 5 by from Staining was fair in IF with HeLa Staining was fair in IF with HeLa. -SCBT QC
Date published: 2015-03-11
Rated 4 out of 5 by from WB: Strong band in HepG2 and Jurkat WB: Strong band in HepG2 and Jurkat. -SCBT QC
Date published: 2014-06-23
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