Anti-alpha 1b Tubulin Antibody (1H10) recommended for detection of α1b Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-α1b Tubulin Antibody (1H10): sc-134238

Anti-α1b Tubulin Antibody (1H10) is rated 2.8 out of 5 by 4.
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Datasheets

    • α1b Tubulin Antibody (1H10) is a mouse monoclonal IgG2b (kappa light chain) provided at 100 µg/ml
    • raised against recombinant α1b Tubulin protein of human origin
    • recommended for detection of α1b Tubulin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α1b Tubulin (1H10): sc-134238.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for α1b Tubulin Antibody (1H10). This work is in progress.
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Can sc-134238: α1b Tubulin (1H10) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, sc-134238: α1b Tubulin (1H10) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good for WB I tested a trial sample of this Ab, it looks useful for WB but not excellent.
Date published: 2017-11-03
Rated 1 out of 5 by from No special band I tried it for 3 times, either no special band or no any band.
Date published: 2017-09-23
Rated 3 out of 5 by from Works for paraffin sections Works for paraffin sections, liked to have seen stronger staining with human prostate samples. -SCBT QC
Date published: 2015-03-16
Rated 3 out of 5 by from WB: Band at correct size but faint in A WB: Band at correct size but faint in A-431. -SCBT QC
Date published: 2014-04-14
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