Date published: 2025-9-5
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alpha Tubulin Antibody (B-7) - Western Blotting - Image 394253
α 管蛋白抗体 (B-7) Alexa Fluor® 647: sc-5286 AF647. 直接荧光西部印迹分析 HeLa (A) 和 HEL 92. 1. 7 (B) 全细胞裂解液中 α 管蛋白的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 488 检测 Cruz Marker™ 分子量标准: sc-516790.
α 管蛋白抗体 (B-7) Alexa Fluor® 647: sc-5286 AF647. 直接荧光西部印迹分析 HeLa (A) 和 HEL 92. 1. 7 (B) 全细胞裂解液中 α 管蛋白的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 488 检测 Cruz Marker™ 分子量标准: sc-516790.

alpha Tubulin 抗体 (B-7): sc-5286

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说明书
  • alpha Tubulin抗体(B-7)是小鼠单克隆IgG2a κ, 在1,631篇文献中引用，规格为200 µg/ml
  • 免疫human物种的α Tubulin的氨基酸149-448
  • 抗-alpha Tubulin 抗体 (B-7) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat 和human 来源的 α Tubulin; 也和以下物种反应，包括： 和 canine, bovine and porcine
  • 抗alpha Tubulin抗体（B-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取alpha Tubulin (B-7): sc-5286的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 alpha Tubulin 抗体 (B-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与alpha Tubulin 抗体 (B-7) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Tubulin 抗体 包括 α 1 Tubulin, α 3 Tubulin, α 4 Tubulin, α Tubulin, β Tubulin, β 2 Tubulin, β 3 Tubulin, δ Tubulin, ε Tubulin和γ Tubulin

α Tubulin 抗体（B-7）是一种小鼠单克隆 IgG2a kappa 轻链抗体，针对人类 α Tubulin 氨基酸 149-448 进行免疫。抗-α Tubulin 抗体（B-7）可检测多种物种的 α Tubulin ，包括小鼠、大鼠和人类，可用于蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）等应用。此外，α微管蛋白单克隆抗体（B-7）与其他物种（包括犬、牛和猪）存在交叉反应。微管蛋白是细胞骨架的重要组成部分，在细胞结构、形状和运动中起着核心作用。α微管蛋白有五种不同的形式：α、β、γ、δ和ε微管蛋白，其中α和β微管蛋白形成异二聚体，并组装成微管。这些微管对于细胞的各种过程至关重要，包括有丝分裂、细胞内运输和维持细胞完整性。微管蛋白在细胞内运输中的作用对于将细胞器和囊泡输送到特定位置至关重要，从而确保细胞正常功能和响应环境信号。此外，参与有丝分裂纺锤体的形成对于精确的染色体分离至关重要，突出了在细胞分裂过程中维持基因组稳定性的重要性。α 管蛋白（B-7）抗体也有多种结合形式，以满足特定实验需求，例如辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

alpha Tubulin 抗体 (B-7) 参考文献:

  1. 乙酰化的α-微管蛋白K394调节微管的稳定性，从而影响轴突末梢的生长。  |  Saunders, HAJ., et al. 2022. Curr Biol. 32: 614-630.e5. PMID: 35081332
  2. 芦丁通过组蛋白去乙酰化酶6的抑制作用增加α-微管蛋白的乙酰化作用。  |  Çetin, Ö., et al. 2022. Drug Dev Res. 83: 993-1002. PMID: 35266183
  3. α-微管蛋白赖氨酸40上的乙酰化作用控制着心脏对葡萄糖的摄取。  |  Renguet, E., et al. 2022. Am J Physiol Heart Circ Physiol. 322: H1032-H1043. PMID: 35486479
  4. α-微管蛋白去乙酰化作用将MCAK活性的抑制与紫杉醇的细胞毒性联系起来。  |  Lopes, D., et al. 2023. J Cell Biol. 222: PMID: 36459065
  5. 微管蛋白同型调节维持α-微管蛋白对β-微管蛋白的不对称需求。  |  Wethekam, LC. and Moore, JK. 2023. J Cell Biol. 222: PMID: 36719400
  6. α-微管蛋白同型微管的冷冻电子显微镜显示α4A/β2A微管的收缩结构。  |  Diao, L., et al. 2023. Acta Biochim Biophys Sin (Shanghai). 55: 1551-1560. PMID: 37439022
  7. 啤酒酵母中5'内含子对α-微管蛋白的调节。  |  Wethekam, LC. and Moore, JK. 2023. Genetics. 225: PMID: 37675603
  8. α-微管蛋白特异性和修饰状态依赖性谷氨酸化作用的结构基础。  |  Mahalingan, KK., et al. 2024. Nat Chem Biol. 20: 1493-1504. PMID: 38658656
  9. MFN2通过α-微管蛋白的乙酰化作用配位线粒体的运动，这种调节作用在CMT2A中受到破坏。  |  Kumar, A., et al. 2024. iScience. 27: 109994. PMID: 38883841
  10. HDAC6催化的α-微管蛋白乳酰化对细胞骨架功能的代谢调节。  |  Sun, S., et al. 2024. Nat Commun. 15: 8377. PMID: 39333081

订购信息

产品名称产品编号规格价格数量收藏夹

alpha Tubulin 抗体 (B-7)

sc-5286
200 µg/ml
$316.00

alpha Tubulin (B-7): m-IgG Fc BP-HRP 套装

sc-528145
200 µg Ab; 10 µg BP
$354.00

alpha Tubulin (B-7): m-IgGκ BP-HRP 套装

sc-520452
200 µg Ab, 40 µg BP
$354.00

alpha Tubulin (B-7): m-IgG2a BP-HRP 套装

sc-547033
200 µg Ab; 10 µg BP
$354.00

alpha Tubulin 抗体 (B-7) AC

sc-5286 AC
500 µg/ml, 25% agarose
$416.00

alpha Tubulin 抗体 (B-7) HRP

sc-5286 HRP
200 µg/ml
$316.00

alpha Tubulin 抗体 (B-7) FITC

sc-5286 FITC
200 µg/ml
$330.00

alpha Tubulin 抗体 (B-7) PE

sc-5286 PE
200 µg/ml
$343.00

alpha Tubulin 抗体 (B-7) Alexa Fluor® 488

sc-5286 AF488
200 µg/ml
$357.00

alpha Tubulin 抗体 (B-7) Alexa Fluor® 546

sc-5286 AF546
200 µg/ml
$357.00

alpha Tubulin 抗体 (B-7) Alexa Fluor® 594

sc-5286 AF594
200 µg/ml
$357.00

alpha Tubulin 抗体 (B-7) Alexa Fluor® 647

sc-5286 AF647
200 µg/ml
$357.00

alpha Tubulin 抗体 (B-7) Alexa Fluor® 680

sc-5286 AF680
200 µg/ml
$357.00

alpha Tubulin 抗体 (B-7) Alexa Fluor® 790

sc-5286 AF790
200 µg/ml
$357.00

alpha Tubulin 抗体 (B-7) TRITC

sc-5286 TRITC
200 µg/ml
$320.00

alpha Tubulin 抗体 (B-7) Alexa Fluor® 405

sc-5286 AF405
200 µg/ml
$357.00

I get two bands on my Western Blot (tissue lysate), one at about 56 kDa and another one at approximately 70 kDa. The upper one is much more equal and seems to be a better loading control. Which one should I use for Quantification?

Asked by: Ann22
Thank you for your question. Please contact our technical service directly for assistance.
Answered by: SCBT Heidelberg Support
Date published: 2025-08-04

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Anna99
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available conjugated binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 5
Date published: 2016-12-16

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Anna99
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal alpha-Tubulin antibody is currently available conjugated to either phycoerythrin (sc-5286 PE), fluoroscein (sc-5286 FITC), Alexa Fluor® 488 (sc-5286 AF488) or Alexa Fluor® 647 (sc-5286 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Tech Service 5
Date published: 2016-12-15
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Rated 5 out of 5 by from Good antibodyThe images of western blot and immunofluorescence are very clear.
Date published: 2023-10-12
Rated 1 out of 5 by from No hubo reconocimiento de la proteinaLo utilicé para detectar tubulina en celulas C6/36 de mosquito pero no obtuve una banda del peso molecuar esperado para la tubulina
Date published: 2023-10-03
Rated 5 out of 5 by from alpha-tubulin antibody, sc-5286The antibody gives strong signals with low background. It works well for Western blotting and immunocytochemistry.
Date published: 2022-12-18
Rated 5 out of 5 by from Good antibodyWe've bought several vials of this antibody to apply to western blot. The signal is clear and strong.
Date published: 2021-10-04
Rated 5 out of 5 by from Strong IF signalsThis antibody gave a clean and strong IF signal in human melanoma cell line MDA-MB-435S and accompanying clones.
Date published: 2021-06-07
Rated 5 out of 5 by from alpha Tubulin antibodyVery useful antibody for loading control in Western blotting.
Date published: 2021-02-10
Rated 4 out of 5 by from Great featuresWe used alpha tubulin antibody for western blot 1:1000, in synaptosomal hippocampal rat brain fraction, and we got clear bands.
Date published: 2020-12-29
Rated 4 out of 5 by from BuonoHo ricevuto il free sample di questo anticorpo alcuni mesi fa. E' stato testato per alcuni western blot e ha funzionato bene.
Date published: 2020-11-05
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alpha Tubulin 抗体 (B-7) is rated 4.6 out of 5 by 52.
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