α-Adaptin 2 Antibody (F-12): sc-55497

α-Adaptin 2 Antibody (F-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects α-Adaptin 2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). α-Adaptin 2 (F-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. α-Adaptin 2 plays a crucial role in the formation of clathrin-coated pits and vesicles, which are essential for receptor-mediated endocytosis, a process vital for cellular uptake of nutrients and signaling molecules. α-Adaptin 2 is primarily located at the plasma membrane and is a key component of the AP-2 adapter complex, which facilitates the transport of vesicles from the plasma membrane. The interaction of α-Adaptin 2 with clathrin and other adaptor proteins ensures the proper assembly and function of these vesicles, highlighting its importance in maintaining cellular homeostasis and regulating intracellular trafficking. Additionally, α-Adaptin 2 has splice variants that exhibit differential expression and localization across various tissues, further underscoring its functional diversity and significance in cellular processes.

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α-Adaptin 2 Antibody (F-12) References:

1. Phylogenetic analysis of components of the eukaryotic vesicle transport system reveals a common origin of adaptor protein complexes 1, 2, and 3 and the F subcomplex of the coatomer COPI.  |  Schledzewski, K., et al. 1999. J Mol Evol. 48: 770-8. PMID: 10229581
2. Interactions of HIV-1 nef with the mu subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based sorting motif.  |  Craig, HM., et al. 2000. Virology. 271: 9-17. PMID: 10814565
3. Numb and alpha-Adaptin regulate Sanpodo endocytosis to specify cell fate in Drosophila external sensory organs.  |  Hutterer, A. and Knoblich, JA. 2005. EMBO Rep. 6: 836-42. PMID: 16113648
4. Conservation and diversity in families of coated vesicle adaptins.  |  Ponnambalam, S., et al. 1990. J Biol Chem. 265: 4814-20. PMID: 1969413
5. Cloning and expression of gamma-adaptin, a component of clathrin-coated vesicles associated with the Golgi apparatus.  |  Robinson, MS. 1990. J Cell Biol. 111: 2319-26. PMID: 2126014
6. Structural and functional division into two domains of the large (100- to 115-kDa) chains of the clathrin-associated protein complex AP-2.  |  Kirchhausen, T., et al. 1989. Proc Natl Acad Sci U S A. 86: 2612-6. PMID: 2495531
7. Cloning of cDNAs encoding two related 100-kD coated vesicle proteins (alpha-adaptins).  |  Robinson, MS. 1989. J Cell Biol. 108: 833-42. PMID: 2564002
8. Structural determinants controlling 14-3-3 recruitment to the endocytic adaptor Numb and dissociation of the Numb·α-adaptin complex.  |  Chen, X., et al. 2018. J Biol Chem. 293: 4149-4158. PMID: 29382713
9. Expression and localization of alpha-adaptin isoforms.  |  Ball, CL., et al. 1995. J Cell Sci. 108 (Pt 8): 2865-75. PMID: 7593326
10. Clathrin assembly protein AP180: primary structure, domain organization and identification of a clathrin binding site.  |  Morris, SA., et al. 1993. EMBO J. 12: 667-75. PMID: 8440257
11. The t(10;11)(p13;q14) in the U937 cell line results in the fusion of the AF10 gene and CALM, encoding a new member of the AP-3 clathrin assembly protein family.  |  Dreyling, MH., et al. 1996. Proc Natl Acad Sci U S A. 93: 4804-9. PMID: 8643484
12. Endocytosis and molecular sorting.  |  Mellman, I. 1996. Annu Rev Cell Dev Biol. 12: 575-625. PMID: 8970738

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-α-Adaptin 2 Antibody (F-12) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-α-Adaptin 2 Antibody (F-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

α-Adaptin 2 Antibody (F-12) Specifications

Alternate Product Name: α-Adaptin 2

Product Clone Name: F-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 678-977

Epitope Target: α-Adaptin 2

Epitope Species: mouse

Product Reactivity/Detection: α-Adaptin 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for α-Adaptin 2 Antibody (F-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred α-Adaptin 1/2 antibody is α-Adaptin 1/2 Antibody (C-8): sc-17771.