Anti-alpha-actinin-4 Antibody (LW-M23) recommended for detection of α-actinin-4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-α-actinin-4 Antibody (LW-M23): sc-134236

Anti-α-actinin-4 Antibody (LW-M23) is rated 4.0 out of 5 by 5.
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Datasheets

    • α-actinin-4 Antibody (LW-M23) is a mouse monoclonal IgG2a κ, cited in 9 publications, provided at 100 µg/ml
    • raised against recombinant α-actinin-4 protein of human origin
    • recommended for detection of α-actinin-4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for α-actinin-4 Antibody (LW-M23). This work is in progress.
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Can sc-134236: α-actinin-4 (LW-M23) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, sc-134236: α-actinin-4 (LW-M23) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good antibody for WB in human Good antibody for WB in human -actinin-4 transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-05-30
Rated 4 out of 5 by from Strong positive band detected via WB in HeLa Strong positive band detected via WB in HeLa nuclear extract. -SCBT QC
Date published: 2015-02-27
Rated 4 out of 5 by from Clear immunoperoxidase nuclear and cytoplasmic Clear immunoperoxidase nuclear and cytoplasmic staining in formalin-fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-02-07
Rated 4 out of 5 by from Clean band seen in WB in A Clean band seen in WB in A-673 whole cell lysate. -SCBT QC
Date published: 2015-01-14
Rated 4 out of 5 by from Good specificity in WB in MCF7 whole cell lysate Good specificity in WB in MCF7 whole cell lysate. -SCBT QC
Date published: 2015-01-14
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