α-actinin-4 Antibody (LW-M23): sc-134236

α-actinin-4 Antibody (LW-M23) is a mouse monoclonal IgG2a antibody that detects α-actinin-4 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). α-actinin-4 is a crucial member of the spectrin gene family, which encodes a diverse group of cytoskeletal proteins, including spectrins, dystrophins, and α-actinins. α-actinin-4 is primarily located in the cytoskeleton of muscle and non-muscle cells, such as skeletal, cardiac, and smooth muscle cells, where α-actinin-4 plays a vital role in maintaining cellular structure and integrity. The unique structure of α-actinin-4, which includes one actin-binding domain, two calponin-homology domains, two EF-hand domains, and four spectrin repeats, allows α-actinin-4 to function effectively as a bundling protein that cross-links filamentous actin (F-actin), anchoring F-actin to various intracellular structures. This anchoring is essential for cellular processes such as muscle contraction and cell signaling. Defects in the gene encoding α-actinin-4 have been linked to focal segmental glomerulosclerosis 1 (FSGS1), a serious renal condition characterized by progressive kidney dysfunction and potential renal failure, highlighting α-actinin-4′s significance in both cellular function and human health.

The preferred α-actinin antibody is α-actinin Antibody (H-2): sc-17829.