Date published: 2026-4-5

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α-Actin Antibody (0.N.5): sc-58669

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Datasheets
  • α-Actin Antibody (0.N.5) is a mouse monoclonal IgG2a κ, cited in 33 publications, provided at 200 µg/ml
  • raised against an N-terminal decapeptide of α-Actin of human origin
  • recommended for detection of smooth muscle α-Actin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including bovine; non cross-reactive with actin from fibroblasts (β-and γ-cytoplasmic), myocardium (α-myocardial), and striated muscle (α-sarcomeric)
  • See α-Actin (1A4): sc-32251 for α-Actin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for α-Actin Antibody (0.N.5) for WB and IHC(P) applications. These reagents are now offered in bundles with α-Actin Antibody (0.N.5) (see ordering information below).
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α-Actin Antibody (0.N.5) is a mouse monoclonal IgG2a antibody that detects α-Actin in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). α-Actin plays a critical role in muscle contraction and cellular structure, as anti-α-Actin antibody (0.N.5) recognizes a key component of the cytoskeleton, providing mechanical support and shape to cells. α-Actin is predominantly found in muscle tissues, where α-Actin monoclonal antibody (0.N.5) detects the contractile apparatus essential for muscle function, and is also present in non-muscle cells, contributing to various cellular processes such as motility and division. The presence of multiple isoforms of Actin, including α-, β-, and γ-Actin, allows for specialized functions in different cell types, with α-Actin being specifically associated with muscle cells. Understanding the dynamics of α-Actin and its interactions with other proteins, such as members of the small GTPase family, is vital for elucidating the mechanisms of cellular movement and stability, making α-Actin (0.N.5) antibody an invaluable tool for researchers studying muscle biology and cytoskeletal dynamics.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

α-Actin Antibody (0.N.5)

sc-58669
200 µg/ml
$322.00

α-Actin Antibody (0.N.5): m-IgG Fc BP-HRP Bundle

sc-539093
200 µg Ab; 10 µg BP
$361.00

α-Actin Antibody (0.N.5): m-IgG2a BP-HRP Bundle

sc-546669
200 µg Ab; 10 µg BP
$361.00

What is the recommended starting dilution for Western blot for sc-58669: α-Actin (0.N.5) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Worked well for immunofluorescenceUsed the antibody at 1:500 dilution for immunofluorescence in FFPE rabbit skin with antigen retrieval performed in a pressure cooker in a sodium citrate pH 6.0 buffer. Successfully stained myofibroblasts in scar tissue, as well as smooth muscle cells in blood vessels.
Date published: 2023-01-14
Rated 5 out of 5 by from immunofluorescenceAntibody was successfully used for immunofluorescence experiments in adipose-derived stem cells at a concentration of 1:2000
Date published: 2019-03-06
Rated 5 out of 5 by from sc-58669 very nice atibody for SM α-ActinI can get very clear bands at 43KDa with 1:10000 dilution of 1st Ab and the same dilution of 2nd Ab. Excellent !
Date published: 2017-08-14
Rated 4 out of 5 by from Good IF with porcine heartGood IF with porcine heart, 1:100 overnight at 4 C. -SCBT Publication Review
Date published: 2011-04-22
Rated 4 out of 5 by from Worked well in paraffinWorked well in paraffin-embedded mouse tissue. 1:25 dilution. -SCBT Publication Review
Date published: 2011-01-11
Rated 5 out of 5 by from Good IF staining inGood IF staining in -20 C methanol fixed PAC1 cells. 1:100 dilution. -SCBT Publication Review
Date published: 2010-12-22
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α-Actin Antibody (0.N.5) is rated 4.7 out of 5 by 6.
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