alpha 3 Sodium Potassium ATPase/ATP1A3 Double Nickase Plasmid (h): sc-416366-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- alpha 3 Sodium Potassium ATPase/ATP1A3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- alpha 3 Sodium Potassium ATPase/ATP1A3 Double Nickase Plasmid (h) and alpha 3 Sodium Potassium ATPase/ATP1A3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ATP1A3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: alpha 3 Sodium Potassium ATPase/ATP1A3 Antibody (H-4): sc-365744
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
alpha 3 Sodium Potassium ATPase/ATP1A3 Double Nickase Plasmid (h) | sc-416366-NIC | 20 µg | $410.00 | |||
alpha 3 Sodium Potassium ATPase/ATP1A3 Double Nickase Plasmid (h2) | sc-416366-NIC-2 | 20 µg | $410.00 |
ATP1A3 encodes the α3 catalytic subunit of the Na⁺/K⁺-ATPase, a P-type ATPase that uses ATP hydrolysis to exchange intracellular Na⁺ for extracellular K⁺, maintaining membrane potential and ionic gradients. This pump supports neuronal excitability, action potential recovery, and secondary active transport processes that couple ion gradients to neurotransmitter uptake and cellular volume regulation. ATP1A3 activity interfaces with pathways governing electrochemical homeostasis, synaptic signaling, and stress responses to metabolic demand in excitable tissues. Genetic and functional perturbations of ATP1A3 are linked to neurological disease phenotypes, making it a key target for mechanistic studies of ion transport dysfunction and circuit-level consequences.
alpha 3 Sodium Potassium ATPase/ATP1A3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ATP1A3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ATP1A3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ATP1A3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ATP1A3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.