α-2M Antibody (H-8): sc-390544

α-2M Antibody (H-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects the alpha-2M protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). α-2M monoclonal antibody (H-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Alpha-2-Macroglobulin (α-2M) is a homotetrameric serum protein composed of four identical subunits that dimerize through disulfide bonds, which is crucial for function as a pan-proteinase inhibitor. This unique structure allows α-2M to "bait" proteinases, leading to a conformational change that effectively traps and inhibits these enzymes, thereby regulating proteolytic activity in the body. Beyond serving as an inhibitor, α-2M also functions as a carrier protein for cytokines during inflammatory responses, binding to transforming growth factor β (TGFβ) and interleukin-6 (IL-6) to modulate activity and concentration in proximity to immune cells. Notably, mutations and deletions in the gene encoding α-2M have been linked to an increased risk of Alzheimer′s Disease, highlighting α-2M′s importance in the clearance of amyloid-beta, a key component in the pathology of this neurodegenerative condition.

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α-2M Antibody (H-8) References:

1. Cloning of the human alpha 2-macroglobulin gene and detection of mutations in two functional domains: the bait region and the thiolester site.  |  Poller, W., et al. 1992. Hum Genet. 88: 313-9. PMID: 1370808
2. Preliminary studies on the interaction of TNF alpha and IFN gamma with alpha 2-macroglobulin.  |  James, K., et al. 1992. Immunol Lett. 32: 49-57. PMID: 1379978
3. Binding of IL-1 beta to alpha-macroglobulins and release by thioredoxin.  |  Borth, W., et al. 1990. J Immunol. 145: 3747-54. PMID: 1700994
4. Sequence analysis of the putative regulatory region of rat alpha 2-macroglobulin gene.  |  Tsuchiya, Y., et al. 1987. Gene. 57: 73-80. PMID: 2448189
5. Natural cytotoxins in human plasma: isolation and characterization of phospholipids associated with cytotoxic lipoproteins.  |  Prezioso, JA. and Koo, PH. 1987. Mol Toxicol. 1: 191-208. PMID: 3449757
6. The interaction of alpha 2-macroglobulin with proteinases. Characteristics and specificity of the reaction, and a hypothesis concerning its molecular mechanism.  |  Barrett, AJ. and Starkey, PM. 1973. Biochem J. 133: 709-24. PMID: 4201304
7. Presence of alpha 2-macroglobulin in normal but not in malignant human syncytiotrophoblasts.  |  Saksela, O., et al. 1981. Cancer Res. 41: 2507-13. PMID: 6165464
8. The proteinase-binding reaction of alpha 2M.  |  Feinman, RD. 1994. Ann N Y Acad Sci. 737: 245-66. PMID: 7524400
9. Altered interaction of Cis-dichlorodiammineplatinum(II)--modified alpha 2-macroglobulin (alpha 2M) with the low density lipoprotein receptor-related protein/alpha 2M receptor but not the alpha 2M signaling receptor.  |  Howard, GC., et al. 1996. J Clin Invest. 97: 1193-203. PMID: 8636430
10. Localization of the binding site for transforming growth factor-beta in human alpha2-macroglobulin to a 20-kDa peptide that also contains the bait region.  |  Webb, DJ., et al. 1998. J Biol Chem. 273: 13339-46. PMID: 9582381
11. Alpha-2 macroglobulin is genetically associated with Alzheimer disease.  |  Blacker, D., et al. 1998. Nat Genet. 19: 357-60. PMID: 9697696
12. [Concentrations of free form and complex form of prostate-specific antigen in serum of alpha 2 macroglobulin deficient patients with prostatic carcinoma].  |  Kanoh, Y., et al. 1998. Rinsho Byori. 46: 923-9. PMID: 9800478

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-α-2M Antibody (H-8) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-α-2M Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

α-2M Antibody (H-8) Specifications

Alternate Product Name: α-2M

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 413-469

Epitope Target: α-2M

Epitope Species: human

Product Reactivity/Detection: α-2M

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for α-2M Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin