α-2M Antibody (3D1): sc-81540

α-2M Antibody (3D1) is a mouse monoclonal IgG1 antibody that detects α-2-Macroglobulin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-α-2M antibody (3D1) recognizes the homotetrameric structure of α-2-Macroglobulin, which consists of four identical subunits that are linked by disulfide bonds to form dimers. This unique structure is crucial as α-2M functions effectively as a pan-proteinase inhibitor, capable of "baiting" proteinases into cleaving specific peptide sequences on its surface. Upon this interaction, α-2M undergoes a conformational change that enables trapping the proteinase, thereby inhibiting its activity and regulating proteolytic processes. Beyond its role as an inhibitor, α-2M also acts as a carrier protein for cytokines during inflammatory responses, binding to transforming growth factor β (TGFβ) and interleukin-6 (IL-6) to modulate their activity and concentration in the vicinity of immune cells. Notably, mutations in the gene encoding α-2M have been linked to an increased risk of Alzheimer′s Disease, highlighting α-2M′s importance in the clearance of amyloid-beta, a key component in the pathology of this neurodegenerative condition. With these multifaceted roles in inflammation and protease regulation, α-2M (3D1) antibody serves as an invaluable tool for researchers studying these critical biological processes.

The preferred α-2M antibody is α-2M Antibody (H-8): sc-390544.