ALKBH6 CRISPR/Cas9 KO Plasmid (h): sc-407122
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ALKBH6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ALKBH6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ALKBH6 CRISPR/Cas9 KO Plasmid (h) | sc-407122 | 20 µg | $397.00 |
Overview
ALKBH6 encodes a member of the AlkB family of Fe(II)/2-oxoglutarate–dependent dioxygenases, a protein group best known for oxidative demethylation chemistry on nucleic acids and related metabolites. Although ALKBH6 remains less well characterized than other ALKBH paralogs, it is studied in the context of cellular responses to alkylation stress, maintenance of genome integrity, and regulation of nucleic acid modification–linked processes. Dysregulation of AlkB-pathway components is frequently investigated for connections to mutational burden, replication stress, and altered RNA/DNA modification landscapes in cancer and other genome-instability–associated conditions. ALKBH6 perturbation models support mechanistic studies of DNA damage response signaling, repair pathway choice, and downstream transcriptional or epitranscriptomic consequences.
ALKBH6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ALKBH6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ALKBH6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ALKBH6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ALKBH6 protein expression.
This CRISPR knockout system enables efficient generation of ALKBH6-deficient cell models for investigation of ALKBH6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ALKBH6 exon(s) critical for ALKBH6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ALKBH6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ALKBH6 CRISPR/Cas9 KO Plasmid (h) and ALKBH6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ALKBH6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ALKBH6 HDR Plasmid (h) and ALKBH6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ALKBH6 homology arms to support homology-directed repair at defined ALKBH6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.